本研究的目标是调查饲料中亚油酸水平和亚油酸：亚麻酸（LA：ALA）比率对生长肥育猪的生长性能、与脂代谢和炎症状态相关基因表达的影响。共有300头生长肥育猪（体重=41.1±6.3 kg）被随机分配到高（30 g/kg；HLA）或低（15 g/kg；LLA）饲料亚油酸水平下，并采用高（23:1；HR）、中等（13:1；MR）或低（4:1；LR）饲料LA：ALA的2 × 3因子设计。饲料在三个28天的阶段内喂养，并以饲料代谢能平衡。猪只在单性别圈舍中以每舍5头的数量养殖。采集d 0、21、42和84血液样本，并从踝关节采集滑膜液进行炎症标志物分析。数据采用PROC MIXED(SAS 9.4)进行重复测量分析，初始体重作为协变量，圈舍作为实验单位，LA水平、LA：ALA、性别、阶段及其交互作用作为固定效应。与HLA相比，LLA猪在d 56和d 84时体重（P = 0.088）略有增加。对于生长性能，LA × LA：ALA没有影响。在整个d 0-84的生长期内，与MR相比，HR饲料的日均增重（ADG）增加，而接受LR饲料的猪只表现介于MR和HR之间。接受HR饲料的母猪与接受低和中等LA：ALA饲料的母猪相比，d 84时体重增加（P = 0.006），这是由于整个d 0-84的ADG改善（P = 0.023）。接受LR饲料的公猪在d 56时体重提高，比MR和HR体重更高，并且在最终体重上比HR更高，MR表现中等（P = 0.006）。这是由于更大的d 0-84的ADG（P = 0.023）。总体而言，猪只血浆中的C-反应蛋白（CRP）、肿瘤坏死因子-α（TNFα）和白细胞介素-6（IL-6）随时间减少（P ≤ 0.037）。在所有处理中，hock和carpus关节液中的CRP和TNFα在d 84与d 0相比减少（P ≤ 0.049）。总之，本研究中使用的LA：ALA比率可以在不影响生长或炎症的情况下喂养不同亚油酸水平的猪只。此外，LA：ALA比率可以对母猪和公猪的生长产生不同影响。© 作者 2023. 由牛津大学出版社代表美国动物科学学会发布。保留所有权利。如需权限，请发送电子邮件至：journals.permissions@oup.com。

The objective of this study was to investigate the effects of dietary linoleic acid level and the ratio of linoleic acid:linolenic acid (LA:ALA) on the growth performance, expression of genes associated with lipid metabolism and inflammatory status of grow-finish pigs. A total of 300 growing pigs (BW = 41.1 ±6.3 kg) were randomly assigned to either a high (30 g/kg; HLA) or low (15 g/kg; LLA) dietary linoleic acid level with a high (23:1; HR), moderate (13:1; MR) or low (4:1; LR) dietary LA:ALA in a 2 × 3 factorial design. Diets were fed across three 28-day phases and were balanced for dietary metabolizable energy. Pigs were housed 5 pigs per pen in single-sex pens. Blood samples were collected on d 0, 21, 42, and 84, and synovial fluid was collected from the hock joint on d 0 and 84 for inflammatory marker analysis. Data were analyzed as repeated measures using PROC MIXED (SAS 9.4) with initial body weight as a covariate, pen as the experimental unit, and LA level, LA:ALA, sex, phases, and their interactions as fixed effects. Compared to HLA, LLA pigs tended to have increased BW at d 56 and d 84 (P = 0.088). There was no effect of LA × LA:ALA for growth performance. For the overall d 0 - 84 growth period, pigs fed HR had increased ADG compared to MR, with pigs receiving LR performing intermediate of MR and HR. Gilts receiving HR diets had increased d 84 BW compared to gilts receiving the low and moderate LA:ALA (P = 0.006), which was a result of improved overall d 0 - 84 ADG compared to gilts receiving the MR diets (P = 0.023). Barrows fed LR had improved BW on d 56 compared to MR and HR and higher final BW compared to HR, with MR performing intermediately (P = 0.006). This was a result of greater d 0 - 84 ADG (P = 0.023). Overall, C-reactive protein (CRP), tumor necrosis factor-α(TNFα), and interleukin-6 (IL-6) were reduced in the plasma of pigs over time (P ≤ 0.037). Across all treatments, CRP and TNFαwere reduced in the hock and carpus synovial fluid on d 84 vs. d 0 (P ≤ 0.049). In conclusion, LA:ALA ratios utilized in this study can be fed at varying linoleic acid levels without impacting growth or inflammation. Additionally, LA:ALA ratios can differentially impact the growth of gilts and barrows.© The Author(s) 2023. Published by Oxford University Press on behalf of the American Society of Animal Science. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.