STAR Related Lipid Transfer Domain Containing 13（STARD13）是一种肿瘤抑制剂，在多种恶性肿瘤中已有所研究。然而，STARD13在调控乳头状甲状腺癌（PTC）进展方面的作用和分子机制仍未被充分探索。我们通过使用“TCGAbiolinks” R软件包下载了甲状腺癌的基因表达和临床信息。利用定量PCR和免疫组织化学染色检测了STARD13在临床肿瘤和相邻非肿瘤样本中的表达情况。通过划痕愈合实验、Transwell实验和3D球体入侵实验评估了PTC细胞的迁移和侵袭能力。细胞增殖能力通过CCK-8实验、克隆形成实验和5-乙炔基-2'-脱氧尿嘧啶（EdU）融入实验进行了测定。Western blotting检测到了所示的蛋白质的变化。 在本研究中，我们发现STARD13在PTC中显著下调，这与预后差相关。STARD13的下调可能是由于启动子区域的甲基化。功能减弱和功能增强实验证明STARD13通过抑制PTC细胞的迁移和侵袭能力在体外和体内起到了作用。此外，我们还发现STARD13调节了PTC细胞的形态并抑制了上皮-间质转化（EMT）。 我们的结果表明，STARD13作为一种转移抑制剂，在PTC中可能是一个潜在的治疗靶点。 © 2023年，作者（S）独家许可Springer Science+Business Media，LLC，SpringerNature公司的一部分。

StAR Related Lipid Transfer Domain Containing 13 (STARD13) serves as a tumor suppressor and has been characterized in several types of malignancies. However, the role and the molecular mechanism of STARD13 in regulating the progression of papillary thyroid carcinoma (PTC) remain underexplored.The gene expression and clinical information of thyroid cancer were downloaded using "TCGAbiolinks" R package. Quantitative PCR and immunohistochemical staining were conducted to detect the expression of STARD13 in clinical tumor and adjacent non-tumor samples. Wound-healing assay, Transwell assay and 3D spheroid invasion assay were performed to evaluate the migratory and invasive capacities of PTC cells. Cell proliferation ability was determined by CCK-8 assay, colony formation assay and 5-ethynyl-2'-deoxyuridine (EdU) incorporation assay. The alterations of indicated proteins were detected by Western blotting.In the present study, we found that STARD13 was significantly underexpressed in PTC, which was correlated with poor prognosis. Downregulation of STARD13 might be due to methylation of promoter region. Loss-and gain-of-function experiments demonstrated that STARD13 impeded migratory and invasive capacities of PTC cells in vitro and in vivo. In addition, we found that STARD13 regulated the morphology of PTC cells and inhibited epithelial-mesenchymal transition (EMT).Our results suggest that STARD13 acts as a metastasis suppressor and might be a potential therapeutic target in PTC.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.