恶性胸膜间皮瘤（MPM）是一种罕见且侵袭性较强的胸膜肿瘤，具有显著的组织学和分子学异质性。初级纤毛（PC），一种在恶性肿瘤中具有新兴重要性的细胞器，在MPM中研究较少。PCR实验室研究了PC功能中关键分子复合物BBSome的表达模式，我们旨在评估其在普通二维和球体三维细胞培养中的表达模式。我们使用了人良性间皮细胞系（MeT-5A）、MPM细胞系（M14K，上皮型MPM；MSTO，两相型MPM）和原发性MPM细胞（pMPM）。设计了特异于人BBS1、2、4、5、7、9、18转录本的引物，并通过实时荧光定量PCR（qRT-PCR）以β-actin为参考基因进行了实验。通过qRT-PCR数据，使用表达因子（1/ΔCt值的均值）分析了2D和3D细胞培养中的相对基因表达。利用2-ΔΔCt方法从qRT-PCR数据中评估了基因表达倍数变化。还通过调节PC的药物硫酸铵（AS）和氯化锂（LC）确定了分子变化。研究中使用的所有细胞大约有15%的区域中存在PC。在所有细胞系和pMPM中，BBSome转录本在细胞培养的不同维度（2D与3D）中表达差异明显。对于良性和MPM细胞，AS和LC处理均影响了纤毛BBS2和BBS18基因的表达。这些数据表明，人类良性和MPM细胞在2D和3D维度的BBSome分子特征有所不同，并支持PC基因应作为潜在的MPM治疗靶点进行研究。© 2023. 作者。

Malignant pleural mesothelioma (MPM), a rare and aggressive pleural tumor, has significant histological and molecular heterogeneity. Primary Cilium (PC), an organelle of emerging importance in malignancies, has been scarcely investigated in MPM. A critical molecular complex for the PC function is the BBSome and here we aimed at assessing its expression patterns in ordinary 2D and spheroid 3D cell cultures.A human benign mesothelial cell line (MeT-5A), MPM cell lines (M14K, epithelioid MPM; MSTO, biphasic MPM), and primary MPM cells (pMPM) were used. Primers specific for the human BBS1, 2, 4, 5, 7, 9, 18 transcripts were designed, and quantitative real-time PCR (qRT-PCR) was done with β-actin as the gene of reference. The relative gene expression across 2D and 3D cultures was analyzed by the expression factor (mean of 1/ΔCt values). With the 2-∆∆Ct method the gene expression fold changes were assessed from qRT-PCR data. Molecular changes using the PC-modulating drugs ammonium sulfate (AS) and lithium chloride (LC) were also determined.PC was present in all cells used in the study at approximately 15% of the observed area. BBSome transcripts were differentially expressed in different dimensions of cell culture (2D vs. 3D) in all cell lines and pMPM. Treatment with AS and LC affected the expression of the ciliary BBS2 and BBS18 genes in the benign as well as in the MPM cells.These data indicate distinct BBSome molecular profiles in human benign and MPM cells cultured in 2D and 3D dimensions and support the notion that PC genes should be investigated as potential MPM therapeutic targets.© 2023. The Author(s).