近年来，盐生微生物代谢物的抗癌性质受到了广泛关注。我们从一个培养基集合中挑选了29株盐生细菌菌种，以评估它们的上清代谢物对六种人类癌细胞系干细胞样特性的影响。用人类成纤维细胞作为正常对照。通过球状体和集落形成实验来评估干细胞样特性。通过入侵和迁移实验以及小鼠模型中的肿瘤发展来评估体外和体内的抗肿瘤作用。来自伊朗盐性细菌Salinivenus iranica的代谢物在所有菌株中对乳腺癌细胞系表现出最强的细胞毒作用（IC50 = 100 µg/mL），对正常细胞没有影响。在MDA-MB-231细胞中，上清代谢物增强了早期和晚期凋亡（分别约为9.5%和48.8%），降低了乳腺癌细胞的球状体和集落形成能力。此外，代谢物的肿瘤内注射后，小鼠模型中的肿瘤发展显著减少，伴随着增加的促凋亡半胱天蛋白酶-3表达。纯化的细胞毒分子为一种分子量为1961.73道尔顿的酚胺（IC50 = 1 µg/mL），能够下调与对传统抗癌治疗产生耐药性相关的多功能基因SRY-Box转录因子2（SOX-2）在乳腺癌细胞中的表达。总之，我们认为来自伊朗盐性细菌Salinivenus iranica的酚胺分子可能是一种潜在的抗乳腺癌成分。© 2023 Springer Nature Limited.

In recent years, the anticancer properties of metabolites from halophilic microorganisms have received a lot of attention. Twenty-nine halophilic bacterial strains were selected from a culture collection to test the effects of their supernatant metabolites on stem cell-like properties of six human cancer cell lines. Human fibroblasts were used as normal control. Sphere and colony formation assay were done to assess the stem cell-like properties. invasion and migration assay, and tumor development in mice model were done to assess the anti-tumorigenesis effect in vitro and in vivo. The metabolites from Salinivenus iranica demonstrated the most potent cytotoxic effect on breast cancer cell lines (IC50 = 100 µg/mL) among all strains, with no effect on normal cells. In MDA-MB-231 cells, the supernatant metabolites enhanced both early and late apoptosis (approximately 9.5% and 48.8%, respectively) and decreased the sphere and colony formation ability of breast cancer cells. Furthermore, after intratumor injection of metabolites, tumors developed in the mice models reduced dramatically, associated with increased pro-apoptotic caspase-3 expression. The purified cytotoxic molecule, a phenol amine with a molecular weight of 1961.73 Dalton (IC50 = 1 µg/mL), downregulated pluripotency gene SRY-Box Transcription Factor 2 (SOX-2) expression in breast cancer cells which is associated with resistance to conventional anticancer treatment. In conclusion, we suggested that the phenol amine molecule from Salinivenus iranica could be a potential anti-breast cancer component.© 2023. Springer Nature Limited.