与成纤维细胞和结肠直肠癌器官样体培养的技术流程.
Protocol for establishing a coculture with fibroblasts and colorectal cancer organoids.
发表日期：2023 Aug 03
作者：
Svenja Wallisch, Sylvia Karin Neef, Lukas Denzinger, Dina Mönch, Jana Koch, Julia Marzi, Thomas Mürdter, Nicole Janssen
来源：
CLINICAL PHARMACOLOGY & THERAPEUTICS
摘要：
肿瘤微环境对于介导耐药性和肿瘤进展至关重要。在这里，我们提出了一种共培养系统，可以在没有额外基质成分如Matrigel或基底膜提取物的情况下，对结直肠癌器官样体和成纤维细胞进行药物测试。首先，我们描述了使用基于发光的细胞活力检测的高通量药物测试的步骤。其次，我们详细介绍了使用流式细胞术来区分对结直肠癌器官样体或成纤维细胞的毒性影响的检测方法。版权所有 © 2023作者. 由Elsevier Inc. 出版，版权所有。
The tumor microenvironment is essential for mediating drug resistance and tumor progression. Here, we present a coculture system, which enables drug testing of colorectal cancer organoids and fibroblasts without additional matrix components such as Matrigel or basement membrane extracts. First, we describe steps to use a readout for high-throughput drug testing using a luminescence-based viability assay. Second, we detail a readout that uses flow cytometry to distinguish toxic effects on either colorectal cancer organoids or fibroblasts.Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.