外泌体表面蛋白可能对乳腺癌的诊断和危险意识具有潜在价值。然而，某些涉及复杂操作和昂贵仪器的检测技术受限于临床应用的推广。为了解决这个问题，我们开发了一种双模式传感器，结合了肉眼检测和电化学分析乳腺癌外泌体表面蛋白的能力。大多数现有传感器依赖于识别外泌体并同时产生放大信号的适配体，这需要设计良好的适配体探针以避免识别外泌体的困难。在我们的研究中，未被外泌体结合的适配体可以作为完整模板诱导G四链体的形成。G四链体/血红素DNA酶的过氧化物酶活性可以催化底物生成颜色和电化学信号。所开发的双模式传感器通过分析外泌体表面蛋白，具有显著区分非转移性乳腺癌患者、转移性乳腺癌患者和健康个体的能力。该传感器的独特特点，包括普适性、简便性和经济性，使其成为乳腺癌研究和临床实践中有希望的诊断工具。 版权所有 © 2023 Elsevier B.V. 保留所有权利。

Exosomal surface proteins are potentially useful for breast cancer diagnosis and awareness of risk. However, some detection techniques involving complex operations and expensive instrumentation are limited to advance to clinical applications. To solve this problem, we develop a dual-modal sensor combining naked-eye detection and electrochemical assay of exosomal surface proteins from breast cancer. Most of existing sensors rely on aptamers recognizing exosomes and generating amplified signals at the same time, which require well-designed aptamer probes to avoid difficulties in identifying exosomes. In our work, aptamers not bound by the exosomes can serve as complete templates to induce formation of G quadruplexes. The peroxidase activity of the G-quadruplex/hemin DNAzyme catalyze substrates can generate both color and electrochemical signals. The developed dual-modal sensor offers a remarkable capability to differentiate nonmetastatic, metastatic breast cancer patients, and healthy individuals through the analysis of exosomal surface proteins. The sensor's distinctive features, including its universality, simplicity, and cost-effectiveness, position it as a promising diagnostic tool in breast cancer research and clinical practice.Copyright © 2023 Elsevier B.V. All rights reserved.