本研究对产DHA微藻Cryptecodinium sp. SUN的细胞外多糖（EPS）进行了特性和抗癌效果的研究。结果显示，C. sp. SUN的EPS分子量为1.118 × 106 g/mol。EPS显著抑制了LA795肺腺癌细胞的增殖和迁移，并且剂量依赖地降低了凋亡率，15 mg/mL时可达到52%。C. sp. SUN的EPS还显著降低了ROS水平超过50%，SOD活性降低了76%，CAT活性降低了34%，说明EPS可能通过抑制肿瘤细胞的生长来抑制肿瘤。此外，C. sp. SUN的EPS通过下调LA795细胞的粘附蛋白和cyclin D1表达来抑制细胞增殖。体内实验证实，C. sp. SUN的EPS抑制了肺腺癌肿瘤的生长，且不影响裸鼠的正常体重。总之，本研究表明C. sp. SUN的EPS可能是一种潜在的癌症治疗物质，为进一步研究EPS提供了研究基础，扩大了Cryptecodinium的应用。版权所有©2023 Elsevier B.V. 发表。

The Characterization and anticancer effects of extracellular polysaccharide (EPS) from DHA-producing microalga Crypthecodinium sp. SUN were studied in the present research. Results showed that EPS from C. sp. SUN have a molecular weight of 1.118 × 106 g/mol. EPS significantly inhibited the proliferation and migration of LA795 lung adenocarcinoma cells, and the apoptosis rate decreased in a concentration-dependent manner, reached 52 % at 15 mg/mL. C. sp. SUN EPS also significantly decreased reactive oxygen species (ROS) level by over 50 %, superoxide dismutase (SOD) activity by 76 %, and catalase (CAT) activity by 34 % at 10 mg/mL, indicating that EPS may inhibit tumor cell growth instead of killing tumor cells. Additionally, C. sp. SUN EPS suppressed cell proliferation by downregulating the expression of adhesion proteins and cyclin D1 in LA795 cells. In vivo experiments demonstrated that C. sp. SUN EPS inhibited the growth of lung adenocarcinoma tumors without affecting the normal body weight of nude mice. Collectively, the present study showed that C. sp. SUN EPS could be a potential substance for cancer treatment, which provided a research basis for future study on EPS and expanded the application of Crypthecodinium.Copyright © 2023. Published by Elsevier B.V.