鸟嘌呤核苷座标修饰通常被认为是非编码RNA中的一种重要表观修饰形式，对于真核生物中的mRNA剪接具有重要作用。该研究通过RNA-Seq技术分析了拟南芥中鸟嘌呤核苷座标修饰与剪接的关系，并发现mRNA中的剪接位点周围富集了鸟嘌呤核苷座标修饰。进一步的研究发现，鸟嘌呤核苷座标修饰可能通过调控RNA的空间结构来影响mRNA的剪接。此外，在剪接因子的结合位点附近也观察到鸟嘌呤核苷座标修饰的存在，这提示鸟嘌呤核苷座标修饰可能通过介导剪接因子的结合来调节剪接的准确性。总之，本研究首次揭示了鸟嘌呤核苷座标修饰介导的mRNA剪接调控网络，为进一步研究RNA剪接提供了重要线索。 鸟嘌呤核苷座位修饰在真核生物中发挥重要作用，并与mRNA剪接紧密相关。本研究对拟南芥中鸟嘌呤核苷座位修饰与剪接的相关性展开了分析，发现mRNA剪接位点周围存在较高水平的鸟嘌呤核苷座位修饰。进一步研究发现，鸟嘌呤核苷座位修饰可能通过影响RNA的空间结构来调节mRNA剪接。此外，鸟嘌呤核苷座位修饰也出现在剪接因子结合位点附近，这提示鸟嘌呤核苷座位修饰可能通过调节剪接因子的结合来影响剪接的准确性。本研究首次揭示了鸟嘌呤核苷座位修饰在mRNA剪接调控中的重要作用，为进一步研究RNA剪接机制提供了新的思路和线索。

Signet-ring cell carcinoma, which is an infrequent type of colorectal cancer. Abdominal pain is the primary presenting complaint of patients with acute appendicitis. It is difficult to diagnose patients with appendiceal carcinomas accompanying with symptoms of acute appendicitis.A 33-year-old female patient was admitted to our hospital, with chief complaints of "bilateral pelvic space-occupying lesions for 1 month, aggravated abdominal distension, and she accompanied with diarrhea for 3 days."The patient was with primary signet ring cell carcinoma of the appendix, presented with acute appendicitis, as well as bilateral ovarian metastasis and peritoneal implantation metastasis.She was then treated with irinotecan, oxaliplatin, calcium folinate, 5-FU combined with bevacizumab, surgical treatment, and postoperative adjuvant treatment with oxaliplatin, capecitabine regimen to consolidate the efficacy.The patient is in good conditions, and postoperative adjuvant chemotherapy is in progress as well.The outcomes highlighted the importance of strict histopathologic assessment for appendiceal adenocarcinoma, and provided new ideas for the diagnosis and treatment of advanced-stage signet ring cell carcinoma of the appendix.Copyright © 2023 the Author(s). Published by Wolters Kluwer Health, Inc.