癌细胞对铁的需求较高，以促进生长和增殖。合成了一种新的铁纳米颗粒和硫脲半胱氨酸酮复合物。确认测试包括紫外可见光谱、扫描电子显微镜（SEM）、能量散射X射线分析（EDX）、傅里叶变换红外光谱（FTIR）、X射线衍射（XRD）和电泳电势测定。MTT测定、流式细胞术和实时定量PCR（qRT-PCR）用于研究抗增殖效应、凋亡数量以及Fe3O4 @Glu/BTSC对microRNA let-7c（let-7c）基因表达的影响。Fe3O4 @Glu/BTSC的规格在5纳米处得到确认。对A549细胞而言，Fe3O4 @Glu/BTSC比BTSC和Fe3O4更有效（IC50=166.77 µg/mL），但其对正常细胞的影响较小（CC50=189.15 µg/mL）。药物选择性指数（SI）计算为1.13。Fe3O4 @Glu/BTSC的初始凋亡率为46.33%，BTSC为28.27%，Fe3O4为26.02%。BTSC和Fe3O4抑制了细胞周期在亚G1和S期的进展。与对照组相比，处理细胞中let-7c表达量增加了6.9倍。与对照组相比，BTSC的表达率为2.2倍，Fe3O4为1.6倍。Fe3O4 @Glu/BTSC通过增加let-7c的表达并抑制细胞周期的凋亡激活途径，对肺癌细胞具有适当的抗增殖效应。©2022作者（们）。本文为开放获取文章，根据创作共用署名4.0许可证（https://creativecommons.org/licenses/by/4.0/）进行使用、传播和再现，只要原始作品得到适当的引用。

Cancer cells have a higher demand for iron to grow and proliferate. A new complex of iron nanoparticles and thiosemicarbazones was synthesized. Confirmation tests included UV-visible, scanning electron microscopy (SEM), energy dispersive X-ray analysis (EDX), Fourier transform infrared (FTIR), X-ray diffraction (XRD) and zeta potential.MTT assay, flow cytometry and qRT-PCR were used to investigate anti-proliferative effect, amount of apoptosis and the effect of Fe3 O4 @Glu/BTSC on changes in gene expression of microRNA let-7c (let-7c), respectively. The specifications of Fe3 O4 @ Glu/BTSC were confirmed at 5 nm.Fe3O4@Glu/BTSC was more effective than BTSC and Fe3 O4 on A549 cells (IC50=166.77 µg/mL) but its effect on healthy cells was smaller (CC50=189.15 µg/mL). The drug selectivity index (SI) was calculated to be 1.13. The initial apoptosis rate was 46.33% for Fe3 O4 @Glu/BTSC, 28.27% for BTSC and 26.02% for Fe3 O4 . BTSC and BTSC@Fe3 O4 inhibited the cell cycle progression in the Sub-G1 and S phases. let-7c expression was 6.9 times higher in treated cells compared to the control group. The expression rate was 2.2 with BTSC compared to the control group and 1.6 times for Fe3 O4.Fe3 O4 @Glu/BTSC has proper anti-proliferative effects against lung cancer cells by increasing the expression of let-7c and inhibiting the cell cycle with the apoptosis activation pathway.© 2022 The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.