新的γ型人乳头瘤病毒（HPV）223号和225号在印度正常和肿瘤参与者的口腔和皮肤中的普遍程度。
Prevalence of novel gamma HPV types 223 and 225 in oral cavity and skin of Indian normal and neoplastic participants.
发表日期：2023 Aug
作者：
Nilanjana Chatterjee, Farhin Sultana, Rituparna Roy, Samyadipta Dey, Sukanya Naskar, Aniruddha Dam, Anup K Bhowmick, Rakiba Begum, Shyam S Mandal, Ranajit K Mandal, Jayanta Chakrabarti, Chinmay K Panda, Massimo Tommasino, Tarik Gheit, Sankhadeep Dutta
来源：
Epigenetics & Chromatin
摘要：
尽管传统上被归类为皮肤病毒，对γ-乳头状瘤病毒（γ-HPV）的实际组织瘤型尚未大量研究。本研究旨在评估两种新型HPV 223和225在226名男性和女性受试者口腔黏膜和角化上皮的组织特异性患病率，这些受试者患有或不患有口腔或子宫颈的肿瘤/ 不典型增生病变。通过聚合酶链反应（PCR）使用针对特定型别的HPV引物检测γ-HPV 223和225 DNA的存在，并通过Sanger测序进行确认。使用绝对实时定量方法确定了HPV 223和HPV 225阳性样本中的病毒载量。为了确定共感染状态，还在口腔黏膜中检测了α-HPV DNA的患病率。新型HPV 223在受试人群的4.4％（10/226）口腔黏膜样本中存在；有趣的是，所有这些样本都是女性，相应的皮肤拭子样本中没有发现患病率。然而，HPV 225的患病率在28.2％（N = 37/131）女性和17.9％（N = 17/95）男性参与者的皮肤和口腔黏膜中发现。此外，发现HPV 223在疾病参与者的口腔黏膜中的病毒载量明显较高（p = 0.02 < 0.05），而HPV 225的病毒载量在正常参与者的口腔黏膜中较高。我们的结果表明，γ-HPV 223仅在口腔黏膜上皮中有患病率，而HPV 225在黏膜和角化皮肤上皮中均有患病率，表明它具有双组织瘤型性质。© 2023 Wiley Periodicals LLC.
Gamma-papillomaviruses, though traditionally classified as cutaneotropic, actual tissue tropism is largely unexplored. This study aimed to evaluate the tissue-specific prevalence of two novel-HPV 223 and 225 in samples of oral mucosa and keratinized epithelium of varied skin parts from 226 female and male subjects, with or without neoplastic/dysplastic lesions in oral cavity or cervix. The gamma-human papillomavirus (gamma-HPV) 223 and 225 DNA presences were determined by polymerase chain reaction (PCR) ursing the HPV type-specific primers and confirmed by Sanger sequencing. Viral load in the HPV 223 and HPV 225 positive samples were determined by absolute real-time quantification method. Alpha-HPV DNA prevalence was also checked in oral mucosa to ascertain coinfection status. Novel HPV 223 was present in 4.4% (10/226) oral mucosal samples of the study population; interestingly all were females with no prevalence in their corresponding skin swab samples. Whereas, the prevalence of HPV 225 was found both in the skin and oral mucosa of 28.2% (N = 37/131) female and 17.9% (N = 17/95) male participants. Alongside, HPV 223 viral load was found to be significantly higher (p = 0.02 < 0.05) in the oral mucosa of diseased participants, whereas, HPV 225 viral load was higher in the oral mucosa of normal participants. Our results suggest that gamma-HPV 223 has its prevalence only in the oral mucosal epithelium, whereas, HPV 225 has its prevalence on both mucosal and keratinized skin epithelium, indicating its dual tropism nature.© 2023 Wiley Periodicals LLC.