本研究探讨了长链非编码RNA BBOX1-AS1对结直肠癌（CRC）在体内和体外的放射治疗敏感性的影响。通过生物信息学数据库和在线预测网站筛选了CRC中差异表达的lncRNA。通过实时定量PCR（RT-qPCR）分析了组织样本中BBOX1-AS1的表达。使用原位荧光杂交（FISH）分析了BBOX1-AS1在CRC细胞中的亚细胞定位。通过Pearson相关系数分析了BBOX1-AS1与CRC组织中PFK1表达水平的相关性。使用RNA和蛋白稳定性测试研究了BBOX1-AS1对PFK1稳定性的影响。使用RNA结合蛋白免疫共沉淀（RIP）和RNA pull-down实验确认了BBOX1-AS1与PFK1的结合。 BBOX1-AS1在CRC中高表达，并与不良预后相关。类似地，它在CRC组织和CRC细胞系中高表达。此外，BBOX1-AS1促进了CRC细胞的增殖、侵袭、迁移和糖酵解，并抑制了细胞凋亡。RIP和RNA pull-down实验证实了BBOX1-AS1与PFK1的结合。RNA稳定性和蛋白稳定性实验证明，BBOX1-AS1影响了PFK1 mRNA和蛋白的稳定性。此外，我们证实了BBOX1-AS1通过调节PFK1的表达增加了放射治疗抗性。 BBOX1-AS1通过稳定PFK1的表达促进了CRC细胞的增殖、侵袭、迁移和糖酵解。BBOX1-AS1还抑制了CRC细胞的凋亡并增加了CRC细胞的放疗抗性。版权所有 © 2023 Elsevier Inc.

Our study explored the effect of long noncoding RNA BBOX1-AS1 on colorectal cancer (CRC) radiosensitivity in vivo and in vitro.Differentially expressed lncRNAs in CRC were screened using a bioinformatics database and an online prediction website. The expression of BBOX1-AS1 in tissue samples was analyzed via real-time quantitative PCR (RT-qPCR). Subcellular localization of BBOX1-AS1 in CRC cells was analyzed using fluorescence in situ hybridization (FISH). The correlation between BBOX1-AS1 and PFK1 expression levels in CRC tissues was analyzed via Pearson's correlation coefficient. The effect of BBOX1-AS1 on PFK1 stability was investigated using RNA and protein stability testing. RNA Binding Protein Immunoprecipitation (RIP) and RNA pull-down assays were used to confirm the binding of BBOX1-AS1 to PFK1.BBOX1-AS1 was highly expressed in CRC and associated with poor prognosis. Similarly, it was highly expressed in CRC tissues and CRC cell lines. In addition, BBOX1-AS1 promoted the proliferation, invasion, migration, and glycolysis of CRC cells and inhibited apoptosis. RIP and RNA pull-down experiments confirmed that BBOX1-AS1 bound to PFK1. RNA stability and protein stability experiments showed that BBOX1-AS1 affected the stability of PFK1 mRNA and protein. Furthermore, we confirmed that BBOX1-AS1 increased radiation resistance through the regulation of PFK1 expression.BBOX1-AS1 promoted the proliferation, invasion, migration, and glycolysis of CRC cells through stabilization of the expression of PFK1. BBOX1-AS1 also inhibited CRC cell apoptosis and increased radiotherapy resistance in CRC cells.Copyright © 2023. Published by Elsevier Inc.