Eleutherine bulbosa (indigenous onion, also known as Dayak onion) extract has been demonstrated to possess notable antioxidant, antitumoral, antimetastatic effects, as well as the capacity to inhibit collagenase enzyme activity. These activities have been evaluated through in vitro, in vivo, and in silico approaches.
Antioxidant, antitumoral, antimetastatic effect and inhibition of collagenase enzyme activity of Eleutherine bulbosa (Dayak onion) extract: In vitro, in vivo and in silico approaches.
发表日期：2023 Aug 04
作者：
Regildo Márcio Gonçalves da Silva, Caio Pismel Alves, Fernando Cesar Barbosa, Hugo Henrique Santos, Kaue Mendonça Adão, Filipe Oliveira Granero, Célia Cristina Malaguti Figueiredo, Carlos Rogério Figueiredo, Nilson Nicolau-Junior, Luciana Pereira Silva
来源：
JOURNAL OF ETHNOPHARMACOLOGY
摘要：
巴西原毛红姜薯（Eleutherine bulbosa (Mill.) Urb.），在巴西被称为“marupazinho”，是一种生长于亚马逊地区的药用植物。该物种的鳞茎传统上以茶或原味（沙拉）的形式用于治疗高血压、糖尿病、胃肠问题、乳腺癌和女性生育力。有报道称该物种拥有具有抗癌作用且作用机制有限的细胞毒性化合物。本研究旨在分析巴西原毛红姜薯鳞茎提取物，并通过体外、体内和体外模拟实验评估其抗氧化活性、抗肿瘤和抗转移作用以及对胶原酶的抑制活性。对总多酚、类黄酮和花青素含量进行了测定，并进行了高效液相色谱 - 质谱分析以鉴定提取物中的植物成分。采用DPPH自由基清除、铁离子还原抗氧化能力（FRAP）、硫代巴比妥酸反应物（TBARS）和一氧化氮（NO）测试进行了抗氧化评估。确定了提取物对小鼠B16F10-Nex2黑色素瘤细胞的抗肿瘤和抗转移活性，并评估了其对胶原酶的抑制活性。还通过分子对接分析评估了化合物与DNA或胶原酶之间的分子相互作用。植物化学评估显示存在多酚、类黄酮和花青素，并且通过HPLC-MS鉴定，结果显示eleutherin、isoeleutherin和eleutherinol的含量较高。抗氧化评估显示提取物在所有评估的方法中均表现出显著活性。体外模拟实验显示活性成分与DNA或胶原酶之间存在相互作用。此外，提取物对黑色素瘤细胞具有抗肿瘤和抗转移作用，并显示出对胶原酶的抑制活性。结果显示，巴西原毛红姜薯鳞茎提取物中含有黄酮类化合物、花青素类和醌类化合物，可能是本研究中观察到的抗氧化、抗肿瘤、抗转移和胶原酶抑制活性的原因。版权所有 © 2023 Elsevier B.V.发表。
Eleutherine bulbosa (Mill.) Urb., known in Brazil as "marupazinho", is a medicinal plant native to the Amazon region. The bulbs of this species are traditionally used in the form of tea or consumed in natura (salads) for the treatment of hypertension, diabetes, gastrointestinal problems, breast cancer, and female fertility. It has been reported that this species possess cytotoxic compounds with anticancer action and limited underlying mechanisms.This study aimed to analyze extract of E. bulbosa bulbs and evaluate antioxidant activity, antitumor and antimetastatic effects against murine B16F10-Nex2 melanoma cells, and collagenase inhibitory activity by in vitro, in vivo, and in silico approaches.Determination of total polyphenols, flavonoids and anthocyanins content were performed. In addition, high performance liquid chromatography-mass spectrometry (HPLC-MS) was carried out to identify phytoconstituents from extract. Antioxidant evaluation was performed using DPPH radical scavenging, ferric ion reducing antioxidant power (FRAP), Thiobarbituric acid reactive species (TBARS) and nitric oxide (NO) tests. Antitumoral and antimetastatic activities of extract on murine B16F10-Nex2 melanoma cells were determined and inhibitory activity on collagenase was evaluated. Molecular interactions between compounds and DNA or collagenase was evaluated by molecular docking analyses.Phytochemical evaluation demonstrated the presence of polyphenols, flavonoids and anthocyanins, and HPLC-MS identified the major presence of eleutherin, isoeleutherin and eleutherinol. Antioxidant evaluation showed that the extract present significant activity in all methods evaluated. In silico assay demonstrated interaction between bioactive compounds and DNA or collagenase. In addition, extract exhibited antitumor and antimetastatic actions promoted by melanoma cells and showed collagenase inhibitory activity.The results showed that E. bulbosa bulb extract contains bioactive compounds as flavonoids, anthocyanins and quinones of which may be responsible for the antioxidant, antitumor, antimetastatic and collagenase enzyme inhibitory activity observed in this study by in vivo, in vitro and in silico bioassays.Copyright © 2023. Published by Elsevier B.V.