组织生物工程、组学和空间生物学领域正快速发展，每个领域都提供了在乳腺癌研究中进行范式转变的机会。然而，目前这些领域之间的合作尚未发挥出其全部潜力。本综述文章中，我们描述了最新生成的关于生物材料和技术平台的三维乳腺癌模型。此外，我们还对这些模型进行了生物学评估，并强调了它们的优点和局限性。具体而言，我们关注最前沿的组学和空间生物学技术，这些技术可以更深入地理解体外培养的生物工程三维乳腺癌模型的生物学相关性，从而为真正具有临床意义的微生理系统、改善药物研发成功率和个体化医学方法铺平道路。版权所有 © 2023 Elsevier Ltd. 保留所有权利。

The fields of tissue bioengineering, -omics, and spatial biology are advancing rapidly, each offering the opportunity for a paradigm shift in breast cancer research. However, to date, collaboration between these fields has not reached its full potential. In this review, we describe the most recently generated 3D breast cancer models regarding the biomaterials and technological platforms employed. Additionally, their biological evaluation is reported, highlighting their advantages and limitations. Specifically, we focus on the most up-to-date -omics and spatial biology techniques, which can generate a deeper understanding of the biological relevance of bioengineered 3D breast cancer in vitro models, thus paving the way towards truly clinically relevant microphysiological systems, improved drug development success rates, and personalised medicine approaches.Copyright © 2023 Elsevier Ltd. All rights reserved.