氧化应激，即过度产生活性氧自由基（ROS），在炎症性疾病的发病机制中起着重要作用，例如心血管疾病、癌症和神经退行性疾病。过氧化氢酶是一种抗氧化酶，具有很大的治疗潜力；然而，其疗效受到将其输送到靶细胞或组织中的限制。为了实现高效输送、一致的药物分布和药物活性，需要小型且尺寸均匀的药物传递载体。在这里，我们使用NanoScribe Photonic Professional GT2高分辨率3D打印机打印了三维（3D）微方体，并研究了3D打印微方体作为过氧化氢酶传递载体的特性。3D打印微方体的尺寸为800纳米的正方形边长和600纳米的高度，非常适合被动地靶向巨噬细胞。微方体的形状和尺寸也是可调节的，高分辨率的3D打印技术可以提供形状和尺寸变化很小的微粒子。过氧化氢酶通过非特异吸附加载到3D打印微方体上，而在3D打印微方体上的过氧化氢酶（CAT-MC）保持了83.1±1.3％的完整酶活性。CAT-MC还能够将巨噬细胞（RAW 264.7）从H2O2的细胞毒性中保护起来，保护率为86.4±4.1％。作为药物传递载体，3D打印的微粒子由于其小巧均匀的尺寸，可以提供一致的药物分布和药物活性，具有很大的前景。因此，我们预期3D打印微粒子在传递治疗蛋白方面将具有广泛的应用。© 2023 The Authors. Published by American Chemical Society.

Oxidative stress, i.e., excessive production of reactive oxygen species (ROS), plays an important role in the pathogenesis of inflammatory diseases such as cardiovascular diseases, cancer, and neurodegenerative diseases. Catalase, an antioxidant enzyme, has great therapeutic potential; however, its efficacy is limited by its delivery to target cells or tissues. In order to achieve efficient delivery, consistent drug distribution, and drug activity, small and uniformly sized drug delivery vehicles are needed. Here, three-dimensional (3D) microcubes were printed by Nanoscribe Photonic Professional GT2, a high-resolution 3D printer, and the characteristics of 3D-printed microcubes as drug delivery vehicles for the delivery of catalase were investigated. The size of the 3D-printed microcubes was 800 nm in length of a square and 600 nm in height, which is suitable for targeting macrophages passively. Microcubes were also tunable in shape and size, and high-resolution 3D printing could provide microparticles with little variation in shape and size. Catalase was loaded on 3D-printed microcubes by nonspecific adsorption, and catalase on 3D-printed microcubes (CAT-MC) retained 83.1 ± 1.3% activity of intact catalase. CAT-MC also saved macrophages, RAW 264.7, from the cytotoxicity of H2O2 by 86.4 ± 4.1%. As drug delivery vehicles, 3D-printed microparticles are very promising due to their small and uniform size, which provides consistent drug distribution and drug activity. Therefore, we anticipate numerous applications of 3D-printed microparticles for delivering therapeutic proteins.© 2023 The Authors. Published by American Chemical Society.