本研究中，我们采用三种溶剂体系对该植物的活性成分进行了仔细提取和鉴定。在提取后，我们使用溶剂体系进一步纯化主要黄酮类化合物。通过我们的分析策略，包括高效液相色谱分析、质谱/质谱光谱分析和核磁共振数据构建，鉴定了黄酮类化合物槲皮素为主要化学成分。我们的研究表明，槲皮素具有治疗乳腺癌细胞系MCF-7和MDA-MB-231的潜在治疗优势，它是在金鳞藤的叶子中发现的化合物。我们将槲皮素与常规处方药多柔比星进行比较，发现它具有抑制MCF-7和MDA-MB-231细胞的能力。细胞凋亡和细胞周期相测量结果证实了这一点。此外，由ROS引起的细胞损伤形成的DNA断裂阶梯进一步证明了药物对DNA完整性的影响。值得注意的是，在处理槲皮素的细胞中，促凋亡蛋白显示出增强的凋亡活性。由于槲皮素是从植物中提取的，并且比其他化合物的副作用更小，它是进一步临床研究的可行选择。目标是对抗全球最常见的疾病之一——乳腺癌，这也是女性死亡的主要原因之一。因此，我们的研究为寻找有效的基于植物的乳腺癌治疗方法做出了重要贡献。在线版本包含附加材料，可在10.1007/s13205-023-03705-w上获取。© 金沙体育投注-官方网站与技术城2023。Springer Nature或其许可方（如学会或其他合作伙伴）根据与作者或其他权利人达成的出版协议独家拥有本文的发表权；作者自行归档接受稿版本的本文仅受此类出版协议和适用法律的约束。

In this study, the active components of the plant were carefully extracted and identified using three solvent systems. After extraction, we used solvent systems to further purify the main flavonoid chemical constituent. As a result of our analytical strategy, which included HPLC analysis, MS/MS spectroscopic analysis, and NMR data-based constructions, quercetin was determined to be the main chemical constituent. Our study suggests the potential therapeutic advantages of quercetin, a compound found in the leaves of Acalypha indica, for treating breast cancer cell lines MCF-7 and MDA-MB-231. Our comparison of Quercetin to the regularly prescribed medicine Doxorubicin shows that it has the capacity to inhibit MCF-7 and MDA-MB-231 cells. Measurements of apoptosis and cell cycle phase showed this to be the case. Furthermore, a ladder that formed as a result of cellular damage brought on by ROS provided further proof of the drug's impact on DNA integrity. Notably, pro-apoptotic proteins displayed increased apoptosis activity in cells treated with quercetin. Given that it is extracted from plants and has less adverse effects than other compounds, quercetin is a viable option for further clinical study. The objective is to fight breast cancer, one of the most prevalent diseases in the world and a main cause of death for women. Thus, our research makes a significant addition to the ongoing search for potent, plant-based breast cancer treatments.The online version contains supplementary material available at 10.1007/s13205-023-03705-w.© King Abdulaziz City for Science and Technology 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.