由壳聚糖、水芹黏液和硒纳米颗粒材料制备出具有强效抗菌和抗癌作用的生物活性纳米复合材料。
Fabrication of bioactive nanocomposites from chitosan, cress mucilage, and selenium nanoparticles with powerful antibacterial and anticancerous actions.
发表日期：2023
作者：
Mohsen M El-Sherbiny, Mohamed I Orif, Mohamed E El-Hefnawy, Sultan Alhayyani, Soha T Al-Goul, Rawan S Elekhtiar, Hoda Mahrous, Ahmed A Tayel
来源：
Frontiers in Microbiology
摘要：
通过提供生物安全和有效的健康保护剂，研究者迫切需要天然的生物活性替代品。创新地利用壳聚糖纳米颗粒（NCT）、水芹籽黏多糖（GCm；Lepidium sativum）和GCm介导的硒纳米颗粒（GCm/SeNPs）来制备新型生物活性天然纳米复合材料（NCs），具有卓越的抗菌活性（对沙门氏菌和金黄色葡萄球菌）和抗癌活性（对CaCo-2和HeLa细胞）。成功利用GCm生成了SeNPs，并从NCT和GCm/SeNPs的相互作用比例中制备了不同的NCs配方，包括T1、T2和T3，其比例分别为2:1、1:1和1:2。合成分子的红外分析显示了相互作用分子之间明显的物理相互作用。SeNPs、T1、T2和T3的分子/NCs的平均粒径和电荷分别为（12.7、316.4、252.8和127.3 nm）和（-6.9、+38.7、+26.2和-25.8 mV）。NCs的生物杀伤评估表明，T1是最强的抗菌制剂，而T3是优越的抗癌合金。这些NCs配方可能超越标准生物杀菌剂的生物杀伤潜力。T1-NC可以在9小时内对挑战的沙门氏菌造成严重的破坏/变形，而T3-NCs在处理的HeLa细胞中引发明显的荧光凋亡迹象。创新设计的生物杀菌天然NCs前景广阔，推荐用于控制致病菌和对抗癌细胞。
版权所有 © 2023 El-Sherbiny, Orif, El-Hefnawy, Alhayyani, Al-Goul, Elekhtiar, Mahrous and Tayel.
Natural bioactive alternatives are the utmost requests from researchers to provide biosafe and effectual health-guarding agents. The biopolymers chitosan nanoparticles (NCT), mucilage of cress seed (GCm; Lepidium sativum), and GCm-mediated selenium nanoparticles (GCm/SeNPs) were innovatively employed for fabricating novel bioactive natural nanocomposites (NCs) with elevated bioactivities as bactericidal (against Salmonella typhimurium and Staphylococcus aureus) and anticancer (against CaCo-2 and HeLa cells). The SeNPs were successfully generated with GCm, and different NCs formulations were fabricated from NCT:GCm/SeNPs amalgam ratios including T1, T2, and T3 with 2:1, 1:1, and 1:2 ratios, respectively. The infrared analysis of synthesized molecules appointed apparent physical interactions among interacted molecules. The average particles' sizes and charges of molecules/NCs were (12.7, 316.4, 252.8, and 127.3 nm) and (-6.9, +38.7, +26.2, and -25.8 mV) for SeNPs, T1, T2, and T3, respectively. The biocidal assessment of NCs indicated that T1 was the strongest antibacterial formulation, whereas T3 was the superior anticancer amalgam. These NCs formulations could exceed the biocidal potentialities of standard biocides. T1-NC could cause severe destructions/deformations in challenged S. typhimurium within 9 h, whereas T3-NCs induced apparent fluorescent apoptosis signs in treated HeLa cells. The prospective applications innovatively designed biocidal natural NCs that are recommended for controlling pathogenic bacteria and fighting cancerous cells.Copyright © 2023 El-Sherbiny, Orif, El-Hefnawy, Alhayyani, Al-Goul, Elekhtiar, Mahrous and Tayel.