本文介绍了一种名为UPIPF（上转换聚多巴胺吲哚菁绿酸聚乙二醇叶酸）的新型多功能系统，用于近红外（NIR）照明下通过上转换发光（UCL）成像癌细胞。该系统通过NIR触发的光动力疗法（PDT）和增强的光热疗法（PTT）的组合表现出对人肝癌（HepG2）癌细胞的高效抑制。首先，利用简单的热分解法合成了上转换纳米颗粒（UCNP）。为了提高它们的生物相容性和水溶性，通过配体交换技术将多巴胺聚合物（PDA）引入UCNP。吲哚菁绿（ICG）分子通过静电吸附的方式附着在UCNP-多巴胺（UCNP@PDAs）复合体的表面，以增强光动力疗法和光热疗法的效果。此外，聚乙二醇（PEG）修饰的叶酸（FA）已经被纳入UCNP-多巴胺-吲哚菁绿（UCNP@PDA-ICGs）纳米颗粒中，以增强它们对癌细胞的靶向能力。这些UCNP的优异UCL特性使得最终的UCNP@PDA-ICG-PEG-FA纳米颗粒（简称为UPIPF）成为高效的抗癌药物传递、实时成像和癌细胞早期诊断的潜在候选物。此外，UPIPF系统展现出PDT辅助的PTT效果，为高效抑制癌细胞提供了便捷的途径（超过99%的细胞被杀死）。制备的UPIPF系统显示出在恶性癌症的早期诊断和同时治疗方面的前景。本文受版权保护，版权所有。

This paper introduces a novel multifunctional system called UPIPF (upconversion-polydopamine-indocyanine-polyethylene-folic) for upconversion luminescent (UCL) imaging of cancer cells using near-infrared (NIR) illumination. The system demonstrates efficient inhibition of human hepatoma (HepG2) cancer cells through a combination of NIR-triggered photodynamic therapy (PDT) and enhanced photothermal therapy (PTT). Initially, upconversion nanoparticles (UCNP) have been synthesized using a simple thermal decomposition method. To improve their biocompatibility and aqueous dispersibility, polydopamine (PDA) has been introduced to the UCNP via a ligand exchange technique. Indocyanine green (ICG) molecules have been electrostatically attached to the surface of the UCNP-polydopamine (UCNP@PDAs) complex to enhance the PDT and PTT effects. Moreover, polyethylene glycol (PEG)-modified folic acid (FA) has been incorporated into the UCNP-polydopamine-indocyanine-green (UCNP@PDA-ICGs) nanoparticles to enhance their targeting capability against cancer cells. The excellent UCL properties of these UCNP enable the final UCNP@PDA-ICG-PEG-FA nanoparticles (referred to as UPIPF) to serve as a potential candidate for the efficient anticancer drug delivery, real-time imaging and early diagnosis of cancer cells. Furthermore, the UPIPF system exhibits PDT-assisted PTT effects, providing a convenient approach for efficient cancer cell inhibition (more than 99% cells were killed). The prepared UPIPF system shows promise for early diagnosis and simultaneous treatment of malignant cancers. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.