尽管三阴性乳腺癌只占乳腺癌的五分之一左右，但其转移和死亡率更高。本研究探讨了紫杉醇和塞来昔布联合治疗对参与三阴性转移乳腺癌细胞凋亡的基因表达的影响。首先培养MDA-MB-231细胞，然后将其分别在特定浓度的塞来昔布（CLX）、紫杉醇（PTX）或二者的联合下处理24至48小时。通过MTT法评估细胞活力。利用实时PCR法评估凋亡相关基因的表达水平。利用蛋白质印迹法评估蛋白表达。CLX和PTX的IC50值分别为73.95 μM和3.15 μM。结果表明，PTX、CLX和PTX + CLX显著（p < 0.05）降低了细胞活力。与PTX治疗相比，联合治疗在两个时间点均显著增加了caspase 3基因的表达，48小时后增加了Bax基因的表达，并在两个时间点明显降低了Bcl-2基因的表达。蛋白质印迹结果与基因的表达一致。这些发现表明，PTX与CLX的联合治疗显著降低了乳腺癌细胞的活力。此外，当与PTX联合使用时，CLX似乎在调节caspase 3、Bax和Bcl-2的表达水平方面是有效的。© 2023. 作者（或作者们）授予施普林格科技有限责任公司其下的施普林格自然出版集团独家许可。

Although triple-negative breast cancer accounts for less than one-fifth of breast cancers, it has a higher rate of metastasis and mortality. This study investigated the effects of combination treatment with paclitaxel and celecoxib on the expression of genes involved in the apoptosis of triple-negative metastatic breast cancer cells. MDA-MB-231 cells were cultured and then treated with certain concentrations of celecoxib (CLX), paclitaxel (PTX), and combination of them for 24 and 48 h. Cell viability was assessed by the MTT method. The real-time PCR method was utilized to assess the expression level of the genes involved in apoptosis. Western blotting was used for evaluating protein expression. IC50 values for CLX and PTX were 73.95 μM and 3.15 μM, respectively. The results demonstrated that PTX, CLX, and PTX + CLX significantly (p < 0.05) reduced cell viability. The comparison of combination treatment with PTX showed a significant increase in caspase 3 gene expression at both time points, in Bax gene expression after 48 h, and a remarkable decrease in Bcl-2 gene expression at both times. Western blotting results were in line with genes' expression. These findings indicate that a combination of PTX and CLX results in a significantly more reduction in cell viability of breast cancer cells. In addition, it seems CLX may be an effective agent in regulating the expression level of caspase 3, Bax, and Bcl-2 when combined with PTX.© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.