脓毒血症是一种由全身性炎症反应综合征引起的急性疾病，其源于感染。治疗脓毒血症严重症状的药物很少。因此，考虑不同的治疗选择非常重要。在本次研究中，我们研究了腹腔结扎和穿孔术（Cecal Ligation and Perforation，CLP）后给予腺苷N1-氧化物（Adenosine N1-oxide，ANO）和吡格列酮（Pioglitazone）对大鼠的影响。我们将它们随机分为四组（n = 10）：A组为对照组，接受生理盐水；B组为CLP手术对照组；C组接受ANO；D组接受Pioglitazone。我们测量了血清中白细胞介素（Interleukin，IL）-6、IL-1β、肿瘤坏死因子-α（Tumor Necrosis Factor Alpha，TNF-α）、一氧化氮（Nitric Oxide，NO），并测量了肝脏和脾脏匀浆液中超氧化物歧化酶（Superoxide Dismutase，SOD）、过氧化氢酶（Catalase，CAT）、丙二醛（Malondialdehyde，MDA）和髓过氧化物酶（Myeloperoxidase，MPO）的含量。我们还调查了脾脏和肝脏中抗氧化酶的含量，最后检测了细胞存活率和大鼠的存活情况。我们还测量了血清中一氧化氮的含量和所有组的大鼠的存活情况。结果表明，这两种药物能够降低大鼠血清中的IL-1β、IL-6、TNF-α和NO，降低肝脏和脾脏匀浆液中的MDA和MPO，然而在接受ANO和吡格列酮的大鼠中，肝脏和脾脏匀浆液中的SOD和CAT显著增加，与CLP组的大鼠相比。接受ANO和吡格列酮的大鼠的细胞存活率和存活情况也显著改善。腺苷N1-氧化物显示出更强和更有效的效果。© 2023 Urmia University. 版权所有。

Sepsis is an acute condition caused by the systemic inflammatory response syndrome to an infection. There are very few drugs that could improve the severe conditions in patients with sepsis. Hence, it is important to consider different treatment options. In this survey, we studied the effect of adenosine N1-oxide (ANO) and pioglitazone on rats with cecal ligation and perforation (CLP). They were randomly divided to four groups (n = 10) including Group A: as control group receiving normal saline, Group B: the rats with CLP as surgical control group, Group C: the rats receiving ANO, and Group D: the rats receiving pioglitazone. Interleukin (IL) -6, IL-1β, tumor necrosis factor alpha (TNF-α), nitric-oxide (NO) in serum blood and superoxide dismutase (SOD), catalase (CAT) malondialdehyde, (MDA) and myeloperoxidase (MPO) in liver and spleen homogenates were measured. The amount of antioxidant enzymes in the spleen and liver, and finally cell viability and rats' survival were investigated. The measurement of blood serum nitric-oxide and survival of all groups of rats were also performed. The results indicated that both drugs could cause a decrease in IL-1β, IL-6, TNF-α and NO in rat blood serum and MDA and MPO in the liver and spleen homogenates, however, a significant increase in SOD and CAT in the liver and spleen homogenates in rats that received ANO and pioglitazone was observed compared to rats with CLP group. Cell viability and rats' survival were significantly improved in rats that received ANO and pioglitazone compared to rats with CLP group. Adenosine N1-oxide showed stronger and more effective effects.© 2023 Urmia University. All rights reserved.