不断增长的证据强烈表明，在膀胱癌患者中，性别差异在发生率和生存率上起着至关重要的作用，男性发病率较高，女性预后较差。然而，性别差异在膀胱癌中的分子基础仍未知。本研究尝试检测对膀胱癌患者性别差异起贡献的关键基因。首先从基因表达纲元数据库(Gene Expression Omnibus, GEO)获得GSE13507的基因表达谱。然后利用R软件筛选出男性和女性之间的差异表达基因(differentially expressed genes, DEGs)。此外，还进行了蛋白质-蛋白质相互作用(PPI)网络分析、Kyoto Encyclopedia of Genes and Genomes (KEGG)、Gene Ontology (GO)和Kaplan-Meier生存分析等。我们发现了6个在膀胱癌患者性别差异中起关键作用的枢纽基因，包括IGF2、CCL5、ASPM、CDC20、BUB1B和CCNB1。我们的分析表明，在患有膀胱癌的女性受试者的肿瘤组织中，CCNB1和BUB1B均上调表达。而其他基因，如IGF2和CCL5，则与膀胱癌男性患者的不良预后相关。此外，我们还发现了三条信号通路（焦点粘附、类风湿关节炎和人类T细胞白血病病毒感染），在膀胱癌与正常样本之间在男女性别中的差异下调。我们的发现表明，性别差异可能调节与膀胱癌发生和预后有关的关键基因的表达。 版权声明：© 2023 Advanced Biomedical Research.

Growing evidence strongly indicates pivotal roles of gender differences in the occurrence and survival rate of patients with bladder cancer, with a higher incidence in males and poorer prognosis in females. Nevertheless, the molecular basis underlying gender-specific differences in bladder cancer remains unknown. The current study has tried to detect key genes contributing to gender differences in bladder cancer patients.The gene expression profile of GSE13507 was firstly obtained from the Gene Expression Omnibus (GEO) database. Further, differentially expressed genes (DEGs) were screened between males and females using R software. Protein-protein interactive (PPI) network analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene Ontology (GO), and Kaplan-Meier survival analyses were also performed.We detected six hub genes contributing to gender differences in bladder cancer patients, containing IGF2, CCL5, ASPM, CDC20, BUB1B, and CCNB1. Our analyses demonstrated that CCNB1 and BUB1B were upregulated in tumor tissues of female subjects with bladder cancer. Other genes, such as IGF2 and CCL5, were associated with a poor outcome in male patients with bladder cancer. Additionally, three signaling pathways (focal adhesion, rheumatoid arthritis, and human T-cell leukemia virus infection) were identified to be differentially downregulated in bladder cancer versus normal samples in both genders.Our findings suggested that gender differences may modulate the expression of key genes that contributed to bladder cancer occurrence and prognosis.Copyright: © 2023 Advanced Biomedical Research.