胶质母细胞瘤（GBM）是最常见的恶性原发性脑肿瘤，目前缺乏有效的治疗方法。在本研究中，我们利用微流控系统合成了用于GBM治疗的二十二碳六烯酸（DHA）脂质体。DHA是一种人类常见的ω3多不饱和脂肪酸，被广泛应用于抑制癌症发展。所采用的微流控装置可以通过调整流速比、流速和脂质浓度来精确调节脂质体的物理化学特性。成功合成了三种不同大小（80nm和130nm）的脂质体，并成功内化到GBM细胞内，并证明了它们可以降低GBM细胞的存活能力。此外，与游离DHA相比，DHA脂质体在通过caspase-3依赖机制诱导细胞凋亡途径方面表现出显著更高的效率。因此，本研究建立的纳米医学平台为ω3脂肪酸脂质体制剂在癌症治疗中的开发提供了新的机会。Copyright © 2023. Elsevier Inc.出版

Glioblastoma (GBM) is the most prevalent malignant primary brain tumor and currently lacks an effective treatment. In this study, we utilized a microfluidic system to synthesize docosahexaenoic acid (DHA) liposomes for GBM therapy. DHA is an omega-3 (ω3) polyunsaturated fatty acid commonly found in human dietary consumption that has demonstrated potential in mitigating cancer development. The microfluidic device employed allowed for precise fine-tuning of the physicochemical properties of liposomes by adjusting the flow rate ratios, flow rates, and lipid concentrations. Three distinct-sized liposomes, ranging from 80 nm and 130 nm, were successfully internalized by GBM cells, and demonstrated the ability to reduce the viability of these cells. Furthermore, DHA liposomes proved significantly more efficient in triggering apoptotic pathways, through caspase-3-dependent mechanisms, in comparison to free DHA. Thus, the nanomedicine platform established in this study presents new opportunities in the development of liposome formulations incorporating ω3 fatty acids for cancer therapy.Copyright © 2023. Published by Elsevier Inc.