人类乳头瘤病毒（HPV）感染是宫颈癌及其前期病变的主要原因。常州地区HPV基因型的总体流行率已有先前报告。然而，多种HPV感染的分布及其在宫颈损伤中的作用尚未得到充分调查。我们旨在评估常州地区人群中多种HPV感染的患病率。此外，我们分析了与E6和E7（E6/E7）mRNA、液基细胞学和宫颈组织病理异常相关的含前五种高流行HPV的多种HPV感染是否与单一感染有更强的相关性。在本研究中，HPV 16、52、58、53和81是最常见的单一和多种感染中的HPV类型。与单一感染相比，包含HPV 16/52/58的多种感染与E6/E7 mRNA的阳性相关密切。除了HPV 16外，包含其余四种常见HPV的多种感染与不明显意义的非典型扁平上皮细胞或更糟糕的情况相比较，具有显著优势。此外，含前五种高流行HPV的多种感染的女性发生宫颈上皮内瘤变II级或更严重的可能性较单一HPV感染的女性更高。我们的结果显示，含前五种高流行HPV的多种HPV感染在宫颈疾病进展中具有优势，应密切监测。这些发现有助于制定常州地区宫颈癌筛查和疫苗接种的区域预防策略。

Human papillomavirus (HPV) infection is the primary cause of cervical cancer and its precursor lesions. The overall prevalence of HPV genotypes in Changzhou has previously been reported. However, the distribution of multiple HPV infections and their roles in cervical injury have less been investigated. We aimed to assess the prevalence of multiple HPV infections among the people in Changzhou. Furthermore, we analyzed whether multiple HPV infections comprising the top five prevalent HPVs were more associated with abnormalities in E6 and E7 (E6/E7) mRNA, liquid-based cytology, and cervical histopathology than a single infection. In the current study, HPV 16, 52, 58, 53, and 81 were the top five prevalent HPV types, both in single and multiple infections. Compared to a single infection, multiple infections containing HPV 16/52/58 were closely linked to positivity for E6/E7 mRNA. In addition to HPV 16, multiple infections containing the remaining top four HPVs conferred a significant advantage on atypical squamous cells of undermined significance or worse in comparison to a single infection. Furthermore, women with multiple infections containing the top five prevalent HPV types were more likely to develop cervical intraepithelial neoplasia grade II or worse than those with a single HPV infection. Our results demonstrate the superiority of multiple HPV infections containing the top five prevalent HPV types in cervical disease progression, which should be closely monitored. These findings are conducive for formulating regional preventive strategies for cervical cancer screening and vaccination in Changzhou.