细胞表面糖类在多种生理和病理过程中发挥着重要作用，其评估对于生物医学和生物技术具有重要意义。在这里，我们提出了一种快速、多功能和单步多色流式细胞术方法，用于评估细胞表面糖类标志物，使用一系列选择性荧光染料偶联的凝集素。该程序能够与传统的多步凝集素染色方法相比，只需要10倍的细胞数量即可同时检测细胞表面糖类。有趣的是，我们使用了这种单步凝集素阵列结合降维算法的概念验证应用来区分不同的肿瘤和免疫细胞种群。此外，该程序还能够揭示出表达差异的T、B和髓系细胞亚群。因此，我们报告了一种快速而多功能的凝集素细胞术方法，可以在不同实验室和核心设施中轻松实施，同时检测活细胞表面的特定糖类修饰。© 作者 2023。由牛津大学出版社发表。保留所有权利。如需授权，请发送电子邮件至：journals.permissions@oup.com。

Cell surface glycans play essential roles in diverse physiological and pathological processes and their assessment has important implications in biomedicine and biotechnology. Here we present a rapid, versatile and single-step multicolor flow cytometry method for evaluation of cell surface glycan signatures using a panel of selected fluorochrome-conjugated lectins. This procedure allows simultaneous detection of cell surface glycans with a 10-fold reduction in the number of cells required compared to traditional multistep lectin staining methods. Interestingly, we used this one-step lectin array coupled with dimension reduction algorithms in a proof-of-concept application for discrimination among different tumor and immune cell populations. Moreover, this procedure was also able to unveil T, B and myeloid cell sub-clusters exhibiting differential glycophenotypes. Thus, we report a rapid and versatile lectin cytometry method to simultaneously detect a particular repertoire of surface glycans on living cells that can be easily implemented in different laboratories and core facilities.© The Author(s) 2023. Published by Oxford University Press. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.