我们提出了一种有效的表皮性卵巢癌器官样体库建立方案，考虑到其临床表现的异质性和高复发率。我们的方案包括并行测试三种培养基，以确定病人特异性的最佳条件。我们描述了组织分离、差异化播种、器官样体培养和体库建立的步骤。我们还概述了固定、包埋和共聚焦显微成像染色的程序。此外，我们证明了在选择的系中，将离体细胞在塑料表面进行短暂培养可以增强器官样体形成的潜力，扩大其应用范围。有关使用和执行该方案的详细信息，请参阅Hoffmann等人[1]。版权所有 © 2023 作者。由Elsevier Inc.出版。版权所有。

We present a protocol for effective biobanking of epithelial ovarian cancer organoids, considering the heterogeneous clinical presentation and high recurrence rates. Our protocol involves parallel testing of three media to identify patient-specific optimal conditions. We describe steps for tissue dissociation, differential seeding, organoid cultivation, and biobanking. We outline procedures for fixation, embedding, and staining for confocal imaging. Furthermore, we demonstrate that brief cultivation of isolates in 2D on plastic enhances organoid-forming potential in selected lines, expanding their application scope. For complete details on the use and execution of this protocol, please refer to Hoffmann et al.1.Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.