用于将人脑器官样体移植到大鼠视皮层以模拟神经修复的方案。
Protocol for human brain organoid transplantation into a rat visual cortex to model neural repair.
发表日期：2023 Aug 15
作者：
Dennis Jgamadze, Paul M Harary, Mackenzie Castellanos, Rachel Blue, Hongjun Song, Guo-Li Ming, H Isaac Chen
来源：
Epigenetics & Chromatin
摘要：
人源干细胞衍生的器官样体是移植性神经修复的有希望的底物。在本文中，我们描述了一种将前脑器官样体移植到受伤的成年大鼠视皮层的方案。该方案包括开颅术、抽吸损伤、器官样体移植和颅盖成形的手术细节。该平台为研究器官样体作为结构移植物在神经修复中的功效提供了有价值的工具。关于该方案的使用和执行的完整细节，请参考Jgamadze等人的文章。Elsevier Inc.发表。
Human stem-cell-derived organoids represent a promising substrate for transplantation-based neural repair. Here, we describe a protocol for transplanting forebrain organoids into an injured adult rat visual cortex. This protocol includes surgical details for craniectomy, aspiration injury, organoid transplantation, and cranioplasty. This platform represents a valuable tool for investigating the efficacy of organoids as structured grafts for neural repair. For complete details on the use and execution of this protocol, please refer to Jgamadze et al.1.Published by Elsevier Inc.