胶质母细胞瘤（GBM）是成年人中最常见且具有毁灭性的原发性脑肿瘤。这是一种高度致命的疾病，只有25％的患者能存活超过1年，仅有5％的患者能存活超过起病后的5年时间。为了寻找更有效的治疗方法，需要了解肿瘤发生过程的机制。对受肿瘤生长影响的组织的生化异常进行分析可能为解决这个问题提供新的线索。因此，在本研究中，我们应用傅立叶变换红外光谱（FTIR）和拉曼显微光谱技术评估大鼠脑组织中生物分子分布和结构的变化，这些变化是由胶质母细胞瘤发展所导致的。同时，我们还利用同步辐射X射线荧光显微镜确定神经组织中出现的元素异常。为了达到研究的预定目标，我们使用了GBM动物模型。我们将胶质瘤细胞以不同侵袭程度进行颅内植入。对于光谱学研究，我们使用取自癌细胞植入区域的脑片进行实验。所获得的结果揭示了脂质和含酮基化合物含量的下降，蛋白质的组成和结构变化以及肿瘤区域低原子序数元素分布的异常等。Copyright © 2023 Elsevier B.V. All rights reserved.

Glioblastoma multiforme (GBM) is the most common and devastating primary brain tumor among adults. It is highly lethal disease, as only 25% of patients survive longer than 1 year and only 5% more than 5 years from the diagnosis. To search for the new, more effective methods of treatment, the understanding of mechanisms underlying the process of tumorigenesis is needed. The new light on this problem may be shed by the analysis of biochemical anomalies of tissues affected by tumor growth. Therefore, in the present work, we applied the Fourier transform infrared (FTIR) and Raman microspectroscopy to evaluate changes in the distribution and structure of biomolecules appearing in the rat brain as a result of glioblastoma development. In turn, synchrotron X-ray fluorescence microscopy was utilized to determine the elemental anomalies appearing in the nervous tissue. To achieve the assumed goals of the study animal models of GBM were used. The rats were subjected to the intracranial implantation of glioma cells with different degree of invasiveness. For spectroscopic investigation brain slices taken from the area of cancer cells administration were used. The obtained results revealed, among others, the decrease content of lipids and compounds containing carbonyl groups, compositional and structural changes of proteins as well as abnormalities in the distribution of low atomic number elements within the region of tumor.Copyright © 2023 Elsevier B.V. All rights reserved.