N6-甲基腺苷（m6A）是真核转录组中最常见的修饰，对编码和非编码RNA具有广泛的功能。它影响修饰RNA的命运，包括稳定性、剪接和翻译，在转录后调控中发挥重要作用。骨骼在支持和保护肌肉和其他器官、促进有机体运动、确保血液生产等方面起着关键作用。骨骼疾病如骨关节炎、骨质疏松症和骨肿瘤是严重的公共卫生问题。骨发育和成骨分化过程需要通过组蛋白、DNA和RNA修饰等表观遗传机制对基因表达进行精确调控。作为一种可逆的动态表观遗传标记，m6A修饰影响几乎所有重要的生物过程、细胞组分和分子功能，包括骨骼发育和稳态。近年来的研究表明，m6A修饰参与了骨骼发育和骨相关疾病的过程。在本综述中，我们总结了参与RNA m6A修饰的蛋白质以及解析m6A修饰在骨形成和干细胞定向分化中的调控作用的最新进展。我们还讨论了m6A修饰在骨质疏松症和骨肉瘤等骨相关疾病中的病理作用和潜在分子机制，并提出了一些可能的新策略领域，用于预防或治疗骨缺陷和骨疾病。© 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

N6-methyladenosine (m6A) is the most prevalent modification in the eukaryotic transcriptome and has a wide range of functions in coding and noncoding RNAs. It affects the fate of the modified RNA, including its stability, splicing, and translation, and plays an important role in post-transcriptional regulation. Bones play a key role in supporting and protecting muscles and other organs, facilitating the movement of the organism, ensuring blood production, etc. Bone diseases such as osteoarthritis, osteoporosis, and bone tumors are serious public health problems. The processes of bone development and osteogenic differentiation require the precise regulation of gene expression through epigenetic mechanisms including histone, DNA, and RNA modifications. As a reversible dynamic epigenetic mark, m6A modifications affect nearly every important biological process, cellular component, and molecular function, including skeletal development and homeostasis. In recent years, studies have shown that m6A modification is involved in osteogenesis and bone-related diseases. In this review, we summarized the proteins involved in RNA m6A modification and the latest progress in elucidating the regulatory role of m6A modification in bone formation and stem cell directional differentiation. We also discussed the pathological roles and potential molecular mechanisms of m6A modification in bone-related diseases like osteoporosis and osteosarcoma and suggested potential areas for new strategies that could be used to prevent or treat bone defects and bone diseases.© 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.