目的：本研究通过增强干细胞存活和促进血管化组织工程骨，改进了体内生物反应器（IVB）用于骨再生。方法：将12只新西兰兔植入带有兔骨髓间充质干细胞（BMSC）的β-TCP支架。利用微CT、Microfil灌注、组织学染色和RT-PCR检测基因表达，比较灌注IVB与对照组。结果：灌注IVB显示出良好的生物相容性，在兔子中增加了新生骨组织、新生血管化以及上调成骨和血管生成相关基因的表达（p < 0.05）。结论：灌注IVB对于骨再生和组织工程在骨科和颌面外科有很大的潜力。

Aim: This study improved the in vivo bioreactor (IVB) for bone regeneration by enhancing stem cell survival and promoting vascularized tissue-engineered bone. Methods: 12 New Zealand rabbits received β-TCP scaffolds with rabbit bone mesenchymal stem cells (BMSCs) implanted. Perfusion IVB with a perfusion electronic pump was compared with the control group using micro-CT, Microfil perfusion, histological staining and RT-PCR for gene expression. Results: Perfusion IVB demonstrated good biocompatibility, increased neoplastic bone tissue, neovascularization and upregulated osteogenic and angiogenesis-related genes in rabbits (p < 0.05). Conclusion: Perfusion IVB holds promise for bone regeneration and tissue engineering in orthopedics and maxillofacial surgery.