纳米酶介导的化学动力疗法（CDT）是一种具有很高特异性的新开发的治疗方式。然而，CDT的疗效仍然面临免疫抑制性肿瘤微环境（TME）带来的挑战。因此，将CDT与免疫治疗干预相结合具有重要意义。在这里，我们报道了CpG负载、Cu2+掺杂的双层羟基化合物纳米片（CpG/Cu-LDHs）的设计和制备，作为免疫纳米酶以协同CDT与免疫原性细胞死亡（ICD）激活的局部和系统免疫反应来增强整体抗肿瘤疗效。CpG/Cu-LDHs赋予的协同CDT-免疫效应以及通过CpG/Cu-LDHs细胞内免疫抑制性TME重塑能力导致了对小鼠肿瘤模型中经过处理的原发肿瘤和未经处理的远处肿瘤的有效抑制。因此，通过免疫纳米酶将CDT与ICD驱动的原位疫苗样免疫治疗相协同，提供了一种新的、普适的范例，用于设计高效、特异的抗肿瘤策略，无需外部刺激。版权所有，不得转载。

Nanozymes mediated chemodynamic therapy (CDT) is a newly developed therapeutic modality with high specificity. The efficacy of CDT, however, still confronts challenges from the immune inhibitory tumor microenvironment (TME). It is thus of great significance to synergize CDT with immunotherapeutic interventions. Herein, we report the design and preparation of CpG loaded, Cu2+ doped double layered hydroxides nanosheets (CpG/Cu-LDHs) as immuno-nanozymes to potentiate overall anti-tumor efficacy by synergizing CDT with immunogenic cell death (ICD)-activated local and systemic immune responses. Such cooperative CDT-immuno effect together with immunosuppressive TME remodeling capacity conferred by CpG/Cu-LDHs led to effective suppression of both treated primary tumor and untreated distant tumor on a mouse tumor model. Thereby, synergizing CDT with ICD-driven, in situ vaccine-like immunotherapy by immuno-nanozymes provides a novel and generalized paradigm for devising highly efficient and specific anti-tumor strategy without the use of external stimulations. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.