核霉素（NCL）是一种多功能的磷酸蛋白，主要定位于核仁，但也存在于核浆、细胞质和细胞膜中。NCL的主要功能包括DNA和RNA代谢、基因转录和翻译、核糖体生物合成和mRNA稳定性。有报道称人类NCL（hNCL）的定位与肿瘤恶性程度相关。因此，分析NCL的细胞动态可能是有用的。在本文中，我们描述了一种针对hNCL肽段生成的大鼠单克隆抗体（mAb）6F9A6。这种抗体能够识别内源性的人类、猴、狗和小鼠NCL，并且已经在多种癌细胞系中的免疫荧光染色、免疫沉淀和免疫印迹实验中证明其有效。我们预计mAb 6F9A6在癌细胞中对hNCL的功能分析将会有用。

Nucleolin (NCL) is a multifunctional phosphoprotein that is mainly localized in the nucleolus, but it is also found in the nucleoplasm, cytoplasm, and cell membrane. The principal functions of NCL involve DNA and RNA metabolism, gene transcription and translation, ribosome biogenesis, and mRNA stability. It was also reported that the localization of human NCL (hNCL) is related to tumor malignancy. Therefore, analyzing the cellular dynamics of NCL could be useful. In this article, we describe rat monoclonal antibody (mAb) 6F9A6 that was generated against a hNCL peptide. This mAb recognizes endogenous human, monkey, dog, and mouse NCL and was shown to be useful in immunofluorescence staining, immunoprecipitation, and immunoblotting experiments in several cancer cell lines. We anticipate that the mAb 6F9A6 will be useful for functional analyses of hNCL in cancer cells.