纳米纤维膜（NFMs）具有细胞外基质（ECM）模拟结构和独特的物理特性，因此在生物仿生材料领域获得了广泛关注，用于开发与生理相关的体外器官/组织模型。近年来，NFM制备技术的进展极大地促进了基于NFM的细胞培养平台在构建生理器官/组织模型方面的发展。然而，尽管NFM制备技术的重要性，对该技术及其未来方面的深入讨论是不足的。本综述从电纺技术到后处理技术，全面介绍了当前NFM制备技术的最新进展，用于制造各种类型的基于NFM的细胞培养平台。此外，还讨论了NFM基于器官/组织模型构建中的优势，尤其是在组织屏障模型、球体/器官样体和生物仿生器官/组织构建方面。最后，通过展望NFM的制备和利用方面的挑战和未来发展方向来总结本综述。本文受版权保护。保留所有权利。

Nanofiber membranes (NFMs), which have an extracellular matrix (ECM)-mimicking structure and unique physical properties, have garnered great attention as biomimetic materials for developing physiologically relevant in vitro organ/tissue models. Recent progress in NFM fabrication techniques immensely contributed to the development of NFM-based cell culture platforms for constructing physiological organ/tissue models. However, despite the significance of the NFM fabrication technique, an in-depth discussion of the fabrication technique and its future aspect was insufficient. This review provides an overview of the current state-of-the-art of NFM fabrication techniques from electrospinning techniques to post-processing techniques for the fabrication of various types of NFM-based cell culture platforms. Moreover, the advantages of the NFM-based culture platforms in the construction of organ/tissue models are discussed especially for tissue barrier models, spheroids/organoids, and biomimetic organ/tissue constructs. Finally, the review concludes with perspectives on challenges and future directions for fabrication and utilization of NFMs. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.