滤泡性淋巴瘤（FL）肿瘤微环境中普遍存在T淋巴细胞。然而，T细胞的表型可能有所不同，特定T细胞亚群的盛行可能影响肿瘤生物学和患者生存。因此，我们使用CyTOF对82例FL患者进行了cohort研究，以确定特定的T细胞表型是否与不同的肿瘤微环境和患者预后相关。我们鉴定了四个具有不同T细胞表型的免疫亚群，特定的T细胞亚群的盛行与患者的生存相关。相比增加晚期分化阶段的T细胞，具有早期分化阶段的T细胞增多的患者生存显著更好。具体而言，晚期分化表型的CD57 + TFH细胞显著更多地存在于FL患者早期疾病进展的情况下，与较差的生存相关。单细胞分析（CITE-seq）表明，CD57 + TFH细胞与CD57- TFH细胞在转录组水平上存在明显差异，炎症途径上调，表现出免疫耗竭和易感于凋亡。综上所述，我们的研究结果表明FL患者之间存在不同的肿瘤微环境，与T细胞表型有关，增加CD57+ TFH细胞的盛行与患者的生存不良相关。© 2023. Springer Nature Limited.

T-lymphocytes are prevalent in the tumor microenvironment of follicular lymphoma (FL). However, the phenotype of T-cells may vary, and the prevalence of certain T-cell subsets may influence tumor biology and patient survival. We therefore analyzed a cohort of 82 FL patients using CyTOF to determine whether specific T-cell phenotypes were associated with distinct tumor microenvironments and patient outcome. We identified four immune subgroups with differing T-cell phenotypes and the prevalence of certain T-cell subsets was associated with patient survival. Patients with increased T cells with early differentiation stage tended to have a significantly better survival than patients with increased T-cells of late differentiation stage. Specifically, CD57+ TFH cells, with a late-stage differentiation phenotype, were significantly more abundant in FL patients who had early disease progression and therefore correlated with an inferior survival. Single cell analysis (CITE-seq) revealed that CD57+ TFH cells exhibited a substantially different transcriptome from CD57- TFH cells with upregulation of inflammatory pathways, evidence of immune exhaustion and susceptibility to apoptosis. Taken together, our results show that different tumor microenvironments among FL patients are associated with variable T-cell phenotypes and an increased prevalence of CD57+ TFH cells is associated with poor patient survival.© 2023. Springer Nature Limited.