广泛应用绿色合成策略制备多功能纳米材料。使用韩国红参（人参）根的70%乙醇提取物作为还原剂，通过绿色合成方法制备了平均大小为6.95±2.25nm的金纳米球。采用种子介导合成法以金纳米球为种子制备了Au-Ag双金属纳米颗粒。结果显示，平均大小为80.4±11.9nm的Au-Ag双金属纳米颗粒被成功合成。通过扫描透射电子显微镜、能量色散X射线光谱和元素分布图，发现双金属纳米颗粒具有Au-Ag合金核心和富含Au的外壳。X射线衍射分析确认了Au-Ag双金属纳米颗粒的面心立方结构。通过电感耦合等离子体质谱法检测和分析，确定了Au-Ag双金属纳米颗粒的Ag/Au比例为0.20。金纳米球和Au-Ag双金属纳米颗粒被PEG化、叶酸共轭和接枝到氧化石墨烯上。傅里叶变换红外光谱确认了功能化的成功。Docetaxel被载入用于评估抗癌细胞的体外细胞活力。含有Docetaxel的纳米颗粒的抗癌活性高于不含Docetaxel的对照组。具有PEG化、叶酸共轭和接枝到氧化石墨烯上的Docetaxel载药金纳米球在人类胃腺癌细胞（AGS）上显示出最高的抗癌活性。此外，接枝到氧化石墨烯上和载药Docetaxel诱导了高水平的细胞内活性氧自由基产生。对于化学光热（PTT）抗癌治疗，利用808nm近红外激光照射研究了细胞活力。Docetaxel载药的Au-Ag双金属纳米颗粒在AGS细胞上显示出最高的化学光热抗癌活性。因此，本文报道的新型Docetaxel载药的Au-Ag双金属纳米颗粒在化学光热抗癌治疗中具有潜在的应用价值。© 2023. 韩国药学会。

Green synthesis strategies have been widely applied for the preparation of versatile nanomaterials. Gold nanospheres with an average size of 6.95 ± 2.25 nm were green synthesized by using a 70% ethanol extract of Korean red ginseng (Panax ginseng Meyer) root as a reducing agent. A seed-mediated synthesis was conducted to prepare Au-Ag bimetallic nanoparticles using gold nanospheres as seeds. Remarkably, Au-Ag bimetallic nanoparticles with an average size of 80.4 ± 11.9 nm were synthesized. Scanning transmission electron microscopy, energy dispersive X-ray spectroscopy and elemental mappings revealed bimetallic nanoparticles with Au-Ag alloy core and Au-rich shells. A face-centered cubic structure of Au-Ag bimetallic nanoparticles was confirmed by X-ray diffraction analysis. For Au-Ag bimetallic nanoparticles, the ratio of Ag/Au was 0.20 which was detected and analyzed by inductively coupled plasma-mass spectrometry. Gold nanospheres and Au-Ag bimetallic nanoparticles were functionalized by PEGylation, folic acid conjugation and grafting onto graphene oxide. Finally, docetaxel was loaded for evaluating the in vitro cell viability on cancer cells. Successful functionalization was confirmed by Fourier-transform infrared spectra. The anticancer activity of the docetaxel-loaded nanoparticles was higher than that of their non-docetaxel-loaded counterparts. The highest anticancer activity on human gastric adenocarcinoma cells (AGS) was observed in the docetaxel-loaded gold nanospheres that were functionalized by PEGylation, folic acid conjugation and grafting onto graphene oxide. Additionally, grafting onto graphene oxide and docetaxel loading induced high intracellular reactive oxygen species generation. For chemo-photothermal (PTT) anticancer therapy, cell viability was investigated using near-infrared laser irradiation at 808 nm. The highest chemo-PTT anticancer activity on AGS cells was observed in the docetaxel-loaded Au-Ag bimetallic nanoparticles. Therefore, the newly prepared docetaxel-loaded Au-Ag bimetallic nanoparticles in the current report have potential applications in chemo-PTT anticancer therapy.© 2023. The Pharmaceutical Society of Korea.