化疗是治疗胃癌等多种恶性肿瘤最常用的方法。由于药物递送的非特异性和非靶向性，导致耐药性，并促进了癌症进展、复发和转移。为了解决这个问题，我们进行了一项研究，旨在开发一种新的顺铂（Cisp）负载羟基功能化单壁碳纳米管（OH-SWCNT）纳米载体系统，以选择性地消除胃癌干细胞。据我们了解，这是第一项利用超声波将顺铂负载在羟基功能化单壁碳纳米管表面的非共价相互作用研究。采用紫外-可见光谱、傅里叶变换红外光谱和透射电子显微镜对其进行了物理和形态学表征。观察到OH-SWCNT在3.5、5.5和7.4三种pH值下均能持续和可控释放顺铂。通过使用CD133+和CD44+特异性标记物分离胃癌干细胞，并对其进行鉴定。顺铂负载的OH-SWCNT纳米载体能够限制CD133+和CD44+细胞的自我更新能力，同时减少胃癌干细胞中肿瘤球的数量。AGS细胞的细胞存活率在250 μg/ml浓度下为20%。IC50值在200 μg/ml和250 μg/ml的顺铂负载OH-SWCNT下均小于50% mol/L。我们的研究结果表明，顺铂负载OH-SWCNT纳米载体复合物能够靶向胃癌干细胞，为选择性靶向和高效消除胃癌干细胞提供了潜在策略。这可能是预防胃癌复发和转移、改善胃癌治疗的一种有前景的方法。© 2023 The Authors. Published by Elsevier Ltd.

Chemotherapy is the most commonly used therapeutic method for treating many malignancies including gastric cancer. Due to their non-specific and non-targeted drug delivery, it causes resistance leading to cancer progression, relapse, and metastasis of cancer. To overcome this problem we carried out a study aimed to develop a new cisplatin (Cisp) loaded hydroxyl functionalized single-walled carbon nanotube (OH-SWCNT) nanocarrier system to selectively eliminate gastric cancer stem cells. To our understanding, this is the first study of the non-covalent interaction of cisplatin loaded on the surface of hydroxyl-functionalized single-walled carbon nanotubes by ultrasonication. The physical and morphological characterization was carried out by UV-Vis, FTIR spectroscopy, and TEM. A sustained and controlled release of cisp from OH-SWCNT at all three pHs 3.5, 5.5, and 7.4 was observed. Gastric cancer stem cells were isolated from primary cells and were identified by using CD133+ and CD44+ specific markers. Cisplatin-loaded OH-SWCNT nanocarrier was capable of limiting the self-renewal capacity of both CD133+ and CD44+ populations and also decreasing the number of tumorspheres in gastric CSCs. The cell viability percent of AGS cells was 20% at 250 μg/ml concentration. The IC50 value was less than 50% mol/L at both 200 μg/ml and 250 μg/ml of cisplatin-loaded OH-SWCNT. Our findings suggest that cisplatin-loaded OH-SWCNT nanocarrier complexes could target gastric CSCs and also could provide a potential strategy for selectively targeting and efficiently eliminating gastric CSCs. This could be a promising approach to prevent gastric cancer recurrence and metastasis and also improve gastric cancer therapy.© 2023 The Authors. Published by Elsevier Ltd.