本研究评估了特应性皮炎（AD）患者外周血液中活化的CD4+辅助型T细胞（Th1和Th2）中炎性细胞因子和细胞保护型热休克蛋白（HSP）的表达。该研究是作者进行的初步工作，旨在确定与关键免疫参数之间的相关性，这些参数有潜力用于指导开发药物策略，以改变这些因素以促进患有重要特应性疾病的人群健康免疫谱的恢复。本研究的主要实验策略评估了从21名AD患者和12名年龄和性别匹配的健康对照受试者的外周血单个核细胞（PBMC）中的免疫激活状况，使用诱导表达Th1或Th2细胞因子和血红素氧合酶-1（HO-1）的CD4+ T细胞差异化的免疫激活剂酯（PMA）和离子霉素（PMA/I）进行培养，评估使用FC500 Beckman-Coulter流式细胞仪进行。观察到CD4+ T细胞中细胞因子白细胞介素-4（iIL-4）、白细胞介素-5（iIL-5）、白细胞介素-10（iIL-10）、干扰素-γ（iIFN-g）和肿瘤坏死因子-α（iTNF-α）的表达增加。此外，还评估了AD患者与对照受试者血液中细胞的热休克蛋白（HSP）iHO-1和iHSP-70的表达。本研究表明，AD患者的CD4+ T细胞中既有Th1又有Th2相关细胞因子的表达增加，并且Th1和Th2细胞种群之间存在显著的正相关，从而揭示了AD相关的全身性炎症免疫特征。最后，观察到的iHO-1和iHSP-70表达增加可能代表针对AD相关炎症组织损伤的适应性生理对抗措施，暗示HSP诱导剂可能是管理特应性疾病的有希望的候选药物。版权所有© Bentham Science Publishers；如有任何疑问，请电邮至epub@benthamscience.net。

The present study evaluates expression by activated CD4+ T helper1 (Th1) and T helper 2 (Th2) T lymphocytes of pro-inflammatory cytokines and cytoprotective heat shock proteins (HSPs) in peripheral blood of atopic dermatitis (AD) patients.This research represents preliminary work by the authors to identify correlates between critical immune parameters with the potential to serve as guidelines for the development of pharmacological strategies for altering these factors to promote the restoration of healthy immune profiles in persons afflicted with major atopic diseases. The major experimental strategy used in this research assessed immune activation by peripheral blood mononuclear cells (PBMC) from 21 AD patients and 12 age- and gender-matched healthy control subjects cultured with phorbol myristate acetate (PMA) and ionomycin (PMA/I), which are mutagenic immune activators, to induce expression of pro-inflammatory biomarkers in CD4+ T cells differentiated to express Th1 or Th2 cytokines and heme oxygenase-1 (HO-1) intracellularly (i). Evaluations were performed using an FC500 Beckman-Coulter flow cytometer. Elevated CD4+ T cell expression of cytokines, interleukin-4 (iIL-4), interleukin- 5 (iIL-5), interleukin-10 (iIL-10), interferon-gamma (iIFN-g), tumor necrosis factor-alpha (iTNF-α), were observed.Additionally, the heat shock proteins (HSP) iHO-1 and iHSP-70 were evaluated in cells from the blood of AD patients versus the control subjects. The present study demonstrated an elevated expression of both Th1 and Th2-associated cytokines in CD4+ T cells of AD patients, with a significant direct correlation between Th1 and Th2 cell populations, thus yielding insight into the immune features of the AD-associated systemic inflammatory profile.Finally, the observed increased iHO-1 and iHSP-70 expressions likely represent adaptive physiologic countermeasures to AD-associated inflammatory tissue damage, suggesting that HSP inducers are promising candidates for the management of atopic disorders.Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.