栓皮栎（Quercus acutissima CARR.）在亚洲传统食物和草药民族药学中被广泛使用，其显示出多种功能，如抗痴呆、抗肥胖和抗哮喘。然而，有关栓皮栎改善皮肤问题功效的科学证据有限。乙醇萃取的栓皮栎（EeA）处理通过剂量不致死的方式使人类成人低钙高温（HaCaT）细胞中肿瘤坏死因子（TNF）-α刺激的诱导型一氧化氮合酶（iNOS）、环氧合酶 2（COX2）、单核细胞趋化蛋白-1（MCP-1）和白细胞介素（IL）-8的表达失活。此外，EeA处理剂量依赖性地抑制了HaCaT细胞中由TNF-α诱导的体外产生的高角蛋白，同时阻断了角蛋白-1（CK-1）和角蛋白-5（CK-5）的表达。此外，EeA治疗剂量依赖性地刺激了人类成纤维细胞中透明质酸（HA）的表达。利用制备性高效液相色谱和超高效液相色谱-质谱-质谱分析，已鉴定了亚油酰胺作为EeA的功能成分，并验证了其抗炎特性和增强的HA表达。综上所述，这些结果表明通过抗炎和上调HA产生，EeA补充剂在改善皮肤问题方面具有功效。

Acorn (Quercus acutissima CARR.) has been used in traditional food and medicinal ethnopharmacology in Asia, and it has shown multifarious functions such as antidementia, antiobesity, and antiasthma functions. However, there is limited scientific evidence about the efficacy of acorn for ameliorating skin problems. Treatment with ethanol-extracted acorns (EeA's) ablated the expression of inducible nitric oxide synthase (iNOS), cyclooxygenase 2 (COX2), monocyte chemoattractant protein-1 (MCP-1), and interleukin (IL)-8 stimulated by tumor necrosis factor (TNF)-α in human adult low calcium high temperature (HaCaT) cells under sublethal dosages. In addition, treatment with EeA dose dependently inhibited the ex vivo hyper keratin formation induced by TNF-α in HaCaT cells in conjunction with the blockade of cytokeratin-1 (CK-1) and cytokeratin-5 (CK-5) expression. Moreover, EeA treatment stimulated the expression of hyaluronic acid (HA) expression in human fibroblasts in a dose-dependent manner. Linoleamide was identified as the functional component of EeA using preparative high-performance liquid chromatography and ultra high performance liquid chromatography-mass spectrometry-mass spectrometry analysis, and the anti-inflammatory features and enhanced HA expression were verified. Collectively, these results suggest the efficacy of EeA supplementation in improving skin problems via anti-inflammation and upregulating HA production.