微小核糖核酸(microRNAs, miRNAs)在细胞对外源化合物和毒素的应答中起着重要作用。然而，在拟除虫菊酯农药诱导的癌症进展中，miRNA在其中的作用尚不清楚。本研究旨在探究miR-96-5p在氯氰菊酯诱导的乳腺癌细胞增殖和迁移中的功能。在我们的研究中，我们发现miR-96-5p在氯氰菊酯处理后的MCF-7细胞中表达上调。miR-96-5p促进了MCF-7细胞的增殖和迁移，并伴随细胞增殖、迁移和凋亡相关蛋白的表达变化。本研究还发现，miR-96-5p的直接靶位点为homeobox A5 (HOXA5)。HOXA5沉默具有miR-96-5p抑制的相反效果。总之，这些结果表明miR-96-5p通过靶向HOXA5参与了氯氰菊酯促进乳腺癌细胞的增殖和迁移。

MicroRNAs (miRNAs) are major players in cellular responses to xenobiotic compounds and toxins. However, the role of miRNAs in pyrethroid pesticide-induced cancer progression remains unclear. This study aimed to investigate the function of miR-96-5p in permethrin-induced proliferation and migration in breast cancer cells. In our study, the expression of miR-96-5p was upregulated in permethrin-treated MCF-7 cells. MiR-96-5p promoted MCF-7 cell proliferation and migration, accompanied bychanges in the expression of proteins involved in cell proliferation, migration, and apoptosis. Homeobox A5 (HOXA5) was identified as a direct target of miR-96-5p. HOXA5 silencing had the opposite effects with miR-96-5p inhibition. In conclusion, these results suggest that miR-96-5p is involved in permethrin-promoted proliferation and migration of breast cancer cells by targeting HOXA5.