从药用植物中提取的生物合成纳米银结合物（AgNCs）在生物医药领域得到了广泛的研究。首次使用毛发亚科甘蓝种子乙酸乙酯提取物合成了AgNCs，并评估了其对宫颈和口腔癌的抗增殖和抗血管生成作用。利用紫外-可见分光光度计、动态光散射（DLS）和扫描电子显微镜（SEM）对AgNCs进行了表征。采用MTT、DNA断裂和体外抗氧化酶活性测定研究了抗增殖活性。采用体内鸡绒毛膜（CAM）模型评估了抗血管生成活性。GCMS分析在两种提取物中鉴定了共有11种化合物。合成的AgNCs呈球形，平均粒径为97.4 nm（AgAPE（Sox））和64.3 nm（AgAPE（Mac））。AgNCs对Hep2C和KB细胞具有有效抑制作用。在Hep2C细胞中，AgAPE（Mac）显示出最高的超氧化物歧化酶（SOD）、过氧化氢酶（catalase）、谷胱甘肽-S转移酶（GST）活性和较低的丙二醛（MDA）含量，而AgAPE（Sox）显示出最高的谷胱甘肽（GSH）含量。另一方面，在KB细胞中，AgAPE（Sox）显示出较高的SOD、GST活性、GSH含量和较低的MDA含量，而AgAPE（Mac）显示出最高的过氧化氢酶活性。对接分析显示丁香酚和亚油酸与选择的靶点之间具有最大的结合亲和力。AgAPE（Sox）、AgAPE（Mac）处理明显减少了鸡胚血管的厚度、分支和发芽。这项研究表明，由A. precatorius-derived AgNCs增强了对宫颈和口腔癌以及血管生成的作用，潜在地限制了肿瘤的生长。© 2023. Springer Nature Limited.

Biogenic silver nanoconjugates (AgNCs), derived from medicinal plants, have been widely explored in the field of biomedicines. AgNCs for the first-time were synthesized using ethyl acetate seed extracts of Abrus precatorius and their antiproliferative and antiangiogenic efficacies were evaluated against cervical and oral carcinoma. Ultraviolet-Visible spectrophotometry, dynamic light Scattering (DLS), and scanning electron microscopy (SEM) were used for characterization of AgNCs. Antiproliferative activity was investigated using MTT, DNA fragmentation and in-vitro antioxidant enzyme activity assays. In-vivo chick chorioallantoic membrane (CAM) model was used to evaluate antiangiogenic activity. A total of 11 compounds were identified in both the extracts in GCMS analysis. The synthesized AgNCs were spherical shaped with an average size of 97.4 nm for AgAPE (Sox) and 64.3 nm for AgAPE (Mac). AgNCs possessed effective inhibition against Hep2C and KB cells. In Hep2C cells, AgAPE (Mac) revealed the highest SOD, catalase, GST activity and lower MDA content, whereas AgAPE (Sox) showed the highest GSH content. On the other hand, in KB cells, AgAPE (Sox) exhibited the higher SOD, GST activity, GSH content, and least MDA content, while AgAPE (Mac) displayed the highest levels of catalase activity. Docking analysis revealed maximum binding affinity of safrole and linoleic acid with selected targets. AgAPE (Sox), AgAPE (Mac) treatment profoundly reduced the thickness, branching, and sprouting of blood vessels in the chick embryos. This study indicates that A. precatorius-derived AgNCs have enhanced efficacies against cervical and oral carcinoma as well as against angiogenesis, potentially limiting tumour growth.© 2023. Springer Nature Limited.