胰十二指肠家庭盒1（PDX1）被认为是胰腺发生和β细胞维持中起作用的关键转录因子，是一个被称为“主调控因子”的存在。早期研究已经报告了PDX1在人类胃癌细胞中作为肿瘤抑制因子的功能，可以抑制细胞生长。在这里，我们报告了经纯化的人类PDX1融合蛋白的生物活性，使用了细胞迁移、增殖、细胞周期分析和基因表达等多种实验方法。在癌细胞中，重组PDX1蛋白减少了细胞迁移和增殖，并通过诱导胃癌细胞凋亡阻止了细胞生长。在胰管癌细胞中，应用PDX1蛋白导致胰岛素基因表达的诱导。这些实验结果展示了重组人类PDX1融合蛋白对胃癌和胰腺癌细胞的生物活性，并证明其作为一种生物工具，在揭示其在各种细胞过程中的功能方面具有实用性。© 2023 Wiley Periodicals LLC.

Pancreatic and duodenum homeobox 1 (PDX1) is considered as a pivotal transcription factor that acts as a "master regulator" in pancreatogenesis and maintenance of β-cells. Earlier study has reported that PDX1 also functions as a tumor suppressor in human gastric cancer cells by inhibiting cell growth. Here, we report the bioactivity of the purified human PDX1 fusion protein using various assays like cell migration, proliferation, cell cycle analysis, and gene expression. In cancer cells, recombinant PDX1 protein reduced cell migration and proliferation, and arrested cell growth by inducing apoptosis in gastric cancer cells. In pancreatic ductal cancer cells, the application of the PDX1 protein resulted in the induction of insulin gene expression. The results of these experiments demonstrate the biological activity imparted by recombinant human PDX1 fusion protein on gastric and pancreatic cancer cells and its usefulness as a biological tool to elucidate its function in various cellular processes.© 2023 Wiley Periodicals LLC.