我们在这里报告了一种新的且简易的方法，用于通过完整细胞合成磷脂@gold纳米花(AuNFs)作为一种新型仿生有机-无机混合材料。这种纳米结构最引人注目的特点是其在近红外(NIR)和可见光谱区域具有双吸收峰，使其成为一种潜在的对活性氧(ROS)诱导的细胞凋亡起反应的光敏剂。与之前的研究相比，这里提出的纳米结构在一个反应过程中使用活细胞膜中存在的磷脂(作为供体细胞)进行合成，并可检测到AuNF形成的微观过程。通过透射电子显微镜(TEM)、FT-IR、31P-NMR和UV-Vis光谱对所得到的AuNFs的性质进行了评估。设计的细胞膜基纳米结构看起来像一个完整的细胞，能够与其他细胞(作为目标细胞)相互作用，并能在近红外照射下产生细胞毒性的单线态氧。生成的ROS在启动程序性细胞死亡(凋亡)和癌症光动力疗法(PDT)的进程中起着关键作用。对乳腺癌MCF-7细胞的细胞实验表明，它们可能作为光动力疗法剂具有有效性。版权所有 © 2023，由Elsevier B.V.出版。

We report here a newly and facile synthesis of the phospholipids@gold nanoflowers (AuNFs) from intact cells as a new biomimetic organic-inorganic hybrid. The most appealing feature of this nanostructure is its dual-absorbing peak in near infrared (NIR) and visible region of spectra, which makes them a potential light-sensitive agent for reactive oxygen species (ROS)-induced apoptosis. Here, in contrast to previous studies, proposed nanostructures are synthesized in a one-pot reaction using phospholipids present in living cell membranes (as a donor cell) with detectable micro process of AuNF formation. The properties of the resulting AuNFs were evaluated through transmission electron microscopy (TEM), as well as FT-IR, 31P-NMR spectra and UV-Vis spectroscopy. Designed cell membrane-based nanostructure looks like an intact cell and would be able to interact with other cells (as a target cell) and also capable to produce cytotoxic singlet oxygen under NIR irradiation. Generated ROS act as a key player in initiation of programmed cell death (apoptosis) and progress of cancer photodynamic therapy (PDT). Cellular experiments on breast cancer MCF-7 cells demonstrated that they may be effective as photodynamic therapy agents.Copyright © 2023. Published by Elsevier B.V.