内镜下黏膜下剥离术（ESD）已成为早期食管癌的一线治疗方法。然而，在需要广泛剥离的情况下，ESD通常会导致术后狭窄。基础成纤维细胞生长因子（bFGF）据报道在皮肤创伤修复中具有抗瘢痕效果。我们假设抑制肌成纤维细胞的活化可以预防ESD后的狭窄。我们通过ESD切除了完整的猪食管环周部分。为了研究ESD后bFGF对食管狭窄形成的预防效果，我们在ESD后内镜下将浸泡了bFGF的聚乙二醇酸（PGA）片覆盖在创面上，并通过喷洒纤维蛋白胶固定（PGA+bFGF组），仅将PGA片覆盖在创面上，并通过喷洒纤维蛋白胶固定（PGA组），或未给予任何处理（对照组）。在第22天切除食管后，我们评估了黏膜收缩率。与对照组相比，PGA+bFGF组的食管狭窄显著减少，并且α-SMA和Calponin-1抗体染色区域在PGA+bFGF组和PGA组中明显抑制。PGA+bFGF组的纤维层厚度与其他组相比均匀。因此，PGA+bFGF抑制了急性期异常调节的成纤维细胞的发展，从而实现了均匀的创面愈合。食管ESD后的狭窄与急性期纤维化相关。PGA和bFGF的应用可以抑制急性期肌成纤维细胞的活化，从而预防猪食管的狭窄。© 2023. 作者。

Endoscopic submucosal dissection (ESD) has been the first-line treatment for early-stage esophageal cancer. However, it often causes postoperative stricture in cases requiring wide dissection. Basic fibroblast growth factor (bFGF) reportedly has anti-scarring effects during cutaneous wound healing. We hypothesized that suppressing myofibroblast activation will prevent stricture after esophageal ESD.We resected a complete porcine esophagus circumference section by ESD. To investigate the preventive effect of bFGF on esophageal stricture formation after ESD, we endoscopically applied bFGF-soaked poly-glycolic acid (PGA) sheets onto the wound bed after ESD and fixed them by spraying fibrin glue (PGA + bFGF group), PGA sheets alone onto the wound bed and fixed them by spraying fibrin glue (PGA group), or nothing (control group). After removing the esophagus on day 22, we evaluated the mucosal constriction rate.Compared with those in the control group, esophageal stricture was significantly reduced in the PGA + bFGF group, and the areas stained with α-SMA and calponin-1 antibodies were significantly inhibited in the PGA + bFGF and PGA groups. The thickness of the fibrous layer in the PGA + bFGF group was uniform compared to that of the other groups. Thus, PGA + bFGF inhibited the development of unregulated fibroblasts in the acute phase, leading to uniform wound healing.Stenosis after esophageal ESD is related to fibrosis in the acute phase. Administration of PGA and bFGF suppresses myofibroblast activation in the acute phase, thereby preventing esophageal constriction in pigs.© 2023. The Author(s).