背景：乳腺癌是女性最常见的恶性肿瘤和死亡原因。影响肿瘤转移的因素包括淋巴结受累、肿瘤分化程度低和激素受体表达缺乏、高增殖率和血管生成。本研究通过免疫组化方法使用CD34标记评估微血管密度（MVD），研究乳腺癌中血管生成的程度以及与其他组织病理学参数的关系，并与正常组织进行比较。 材料与方法：共纳入58例乳腺癌病理切片标本。将所有标本切片并进行雌激素受体（ER），孕激素受体（PR），人表皮生长因子受体2（HER 2 / neu），Ki67和CD34的免疫组织化学（IHC）染色。 结果：本研究患者的平均年龄为49.6±10.6岁。统计学分析显示，肿瘤级别（P=0.01）、雌激素受体表达缺失（P=0.008）和孕激素受体表达缺失（P=0.003）与MVD存在显著相关。 结论：由于MVD与肿瘤分级、雌激素受体和孕激素受体缺失之间的关联，该有价值的标记物在乳腺癌患者预后预测中可以发挥重要作用，并且可能为未来的新型靶向治疗提供依据。 版权所有 © 2023 Tehran University of Medical Sciences.

Background: Breast cancer is the most common malignant tumor and cause of death in women. Factors that play role in tumor metastasis are lymph node involvement, lack of tumor differentiation and hormone receptor expression, high proliferation rate, and angiogenesis. In the present study, we tried to evaluate the microvessel density (MVD) using Immunohistochemistry for the CD34 marker to investigate the amount of angiogenesis in breast cancer and its relationship with other histopathological parameters and compare it with normal tissue. Materials and Methods: 58 paraffin-embedded samples of breast cancer were enrolled. All blocks were sectioned and stained for estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor 2(HER 2/neu), ki67, and CD34 by immunohistochemistry (IHC) method. Results: The mean age of patients in this study was 49.6 ± 10.6 years. Statistically, there was a significant relationship between the grade of the tumor (P = 0.01), absence of expression of estrogen receptor (P = 0.008), and progesterone receptor (P = 0.003) with MVD. Conclusion: Due to the association between MVD, tumor grade, and absence of ER and PR expression, this valuable marker can play an important role in the prediction of prognosis in breast cancer patients and can lead to new-targeted therapy in the future.Copyright © 2023 Tehran University of Medical Sciences.