RNA修饰包括m6A、m1A、选择性多腺苷酸化和腺苷酸变异为肌苷酸以参与关键的癌症免疫和癌症过程。然而，RNA修饰编写因子在膀胱癌（BLCA）中的功能尚不清楚。本研究使用无监督聚类分析在BLCA中鉴定了新的RNA修饰编写因子介导的分子亚型。通过单变量Cox分析和最小绝对值收缩和选择算子（LASSO）分析，开发了一种称为WriterScore的相应定量指标。然后，我们对RNA修饰编写因子相关聚类（WriterScore）与BLCA中的免疫特征、经典分子亚型、临床病理特征和治疗选择之间进行了系统分析。最后，我们在多个外部BLCA数据集、盛京医院的临床样本数据集和全癌种数据集中验证了WriterScore。获得了两个RNA修饰编写因子相关聚类和三个差异表达基因（DEG）聚类。这些RNA修饰编写因子相关聚类（WriterScore）与BLCA的免疫特征、经典分子亚型和临床病理特征密切相关。此外，WriterScore能够准确预测BLCA患者的临床结果和免疫治疗效果。我们的研究系统地探究了RNA修饰编写因子的作用，并开发了一个重要的WriterScore指标，以指导BLCA的多种治疗选择，可能为BLCA患者带来一些潜在的益处。版权所有 © 2023 张、陈和温。

RNA modifications, containing m6A, m1A, alternative polyadenylation and adenosine-to-inosine RNA editing, involve in critical cancerous immunity and cancerous processes. However, the functional roles of RNA modification writers in bladder cancer (BLCA) are largely unknown.In this study, unsupervised clustering was used to identify novel RNA modification writers -mediated molecular subtypes in BLCA. A corresponding quantitative indicator called WriterScore was developed using univariate Cox and Least absolute shrinkage and selection operator (LASSO) analysis. Then, we systematically analyzed the correlation between RNA modification writer-related clusters (WriterScore) and immunological characteristics, classical molecular subtypes, clinicopathologic features and treatment options in BLCA. Finally, we validated the WriterScore in multiple other external BLCA datasets, clinical sample dataset in Shengjing Hospital and pancancer.Two RNA modification writer-related clusters and three DEGclusters were obtained. These RNA modification writer-related clusters (WriterScore) were strongly associated with immunological characteristics, classical molecular subtypes, clinicopathologic features of BLCA. Moreover, WriterScore can properly predict the clinical outcomes and immunotherapy of BLCA patients.Our study systematically investigated the role of RNA modification writers and developed a significant WriterScore to guide several treatment options in BLCA, which might bring some potential benefits for BLCA patients.Copyright © 2023 Zhang, Chen and Wen.