生成一株携带婴幼儿白血病相关融合基因KMT2A-AFF1（IMSUTi002-A-2）的人类诱导多能干细胞株。
Generation of a human induced pluripotent stem cell line harboring the infant leukemia-associated fusion gene, KMT2A-AFF1 (IMSUTi002-A-2).
发表日期：2023 Aug 22
作者：
Masayuki Takahashi
来源：
Stem Cell Research & Therapy
摘要：
KMT2A-AFF1（MLL-AF4）是由4号和11号染色体易位（t（4;11））所形成，在婴儿前B淋巴母细胞白血病中特异性地出现，并且是白血病发生的主要驱动因子。在本研究中，建立了一株人诱导多能干细胞（hiPSC）系（IMSUTi002-A-2），它具有同时诱导KMT2A-AFF1和EGFP的可诱导表达功能。这种hiPSC可以成为了解婴儿白血病和白血病发生风险评估的有力模型。版权所有© 2023 作者。由Elsevier B.V.出版。保留所有权利。
KMT2A-AFF1 (MLL-AF4), formed by chromosomal translocation t(4;11), is observed specifically in infant pro-B acute lymphoblastic leukemia, and the main driver of leukemogenesis. In this study, a human induced pluripotent stem cell (hiPSC) line harboring KMT2A-AFF1 (IMSUTi002-A-2), which has the ability to simultaneously induce the doxycycline-inducible expression of KMT2A-AFF1 and EGFP, was established. This hiPSC can be a powerful model for understanding infant leukemia and risk assessment of leukemogenesis.Copyright © 2023 The Author. Published by Elsevier B.V. All rights reserved.