成人最常见的白血病形式是急性白血病。药物分化控制是急性白血病非常关键的治疗方法。然而，目前常用的检测分化控制技术经历了漫长的时间和复杂的验证步骤，阻碍了医学发现的进程：流式细胞术和RT-PCR在成本上非常高昂，虽然精确和高效率，但需要不方便的荧光标记或存在高风险的污染；传统染色会导致细胞死亡，无法进行进一步的药理学测试。对于开发简单、快速和非侵入性的检测药物技术产生了很大兴趣。DC-iDEP是一种新兴的无标记识别技术，利用整个细胞的原生生物物理性质对细胞群体进行排序。在这里，HL-60细胞系被用作研究成为颗粒细胞和药效的分化过程的模型。结果显示，DEP成功通过加权平均的特性因子来检测DMSO促进的HL-60分化程度。该因子与单个细胞的生物物理性质有关，这种性质的积累导致每个具有不同构造的群体之间出现差异。此外，已经研究发现顶果酸能够有效促进DMSO诱导的分化。利用顶果酸引起的变化，首次尝试了基于DEP的HL-60药物筛选应用。建立了一种快速、无标记的药物筛选方法，用于监测HL-60分化为颗粒细胞，并具有很大的药物筛选潜力。本文受版权保护。版权所有。

The most common form of leukemia in adults is acute leukemia. Drug differentiation control is an extremely critical treatment for acute leukemia. Unfortunately, current techniques detecting differentiation control experience long time and complex steps of verification hindering the pace of medicine discovery: flow cytometry and RT-PCR are highly accurate and efficient at a cost of inconvenient fluorescent labeling or a high risk of contamination; conventional staining leads to cell death unavailable for further pharmacological tests. There is a great interest in developing simple, fast and non-invasive techniques to screen medicine. DC-iDEP is an emerging label-free identification technique taking advantage of the whole cell native biophysical property for sorting cell populations. Here, HL-60 cell line has been used as a model to study the differentiation process towards granulocytes and medicine efficacy. The results showed that DEP succeeded in detecting the DMSO promoted HL-60 differentiation degree by the weighted average characterization factor. This factor is related to the single cell biophysical property, which accumulates to generate differences in each population with distinct constitutions. Furthermore, chichoric acid was investigated to be capable of promoting DMSO-induced differentiation efficiently. Using the change induced by chichoric acid, the HL-60 medicine screening application has been first attempted based on DEP. A rapid, label-free medicine screening method has been established to monitor HL-60 differentiation towards granulocyte and has great potential for medicine screening. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.