茶树精油（也称为茶树精油，TTO）是从澳洲茶树（学名为互花白千层）提取的一种精油，在传统医学中被用作药物，并在医药、食品和化妆品行业中用作治疗。然而，这种精油非常不稳定、易挥发和易氧化，这限制了它的实际应用。本研究的目的是合成基于茶树油的油水（O/W）纳米乳液（茶树精油纳米乳液，TNE）并评估其生物学潜力。通过紫外线、荧光和傅里叶红外光谱技术进行了生理特性的表征。还估计了各种生物活性，如抗癌、抗糖尿病和抗炎等。进行了TNE的药代动力学研究。纳米乳液的包封效率为83%。纳米乳液呈球形，粒径为308nm，Zeta电位为-9.42，聚集度指数为0.31。纳米乳液在不同温度下经过50天储存仍然稳定。通过各种测定进行了TNE的抗氧化潜力评估，IC50值分别为：一氧化氮自由基清除活性为225.1，DPPH自由基清除活性为30.66，铁螯合试验为38.73，铁还原试验为39.36。治疗HeLa细胞后，用1μl、2μl和5μl的TNE的细胞存活率分别为82%、41%和24%。抗糖尿病研究表明，TNE以剂量依赖性方式抑制α-淀粉酶，在其较高体积的250µl时，抑制率为88%。药物动力学研究表明，纳米乳液遵循一级动力学模型。基于此，讨论了茶树精油基纳米乳液在化妆品、食品和制药行业中的可能角色。

Essential oil from Melaleuca alternifolia (also known as Tea tree essential oil, TTO) is used as traditional medicine and used as therapeutic in medicine, food and cosmetic sectors. However, this oil is highly unstable, volatile and prone to oxidation which limits its practical use.The objective of this study was synthesis of tea tree oil based O/W (oil/water) nanoemulsions (tea tree essential oil nanoemulsions, TNE) and evaluation of its biological potential. Physiological characterization was carried out using UV, fluorescent, and FT-IR techniques. Various biological activities such as anticancerous, antidiabetic and antiinflammatory were also estimated. Pharmacokinetics study on TNE was carried out. Encapsulation efficiency of nanoemulsions was found to be 83%. Nanoemulsions were spherical in shape with globule size 308 nm, zeta potential -9.42 and polydispersity index was 0.31. Nanoemulsions were stable even after 50 days of storage at different temperatures. Antioxidant potential of TNE was conducted by various assays and IC50 were: Nitric oxide radical scavenging activity:225.1, DPPH radical scavenging activity:30.66, Iron chelating assay:38.73, and Iron reducing assay: 39.36. Notable anticancer activity was observed with the percent cell viability of HeLa cells after treatment with 1 μl, 2 μl and 5 μl of TNE was 82%, 41% and 24%, respectively. Anti-diabetic study revealed that TNE inhibited -amylase in a dose-dependent manner, with 88% inhibition at its higher volume of 250 µl. Drug kinetic study revealed that nanoemulsions exhibited first-order model. Based on this, the possible role of Melaleuca alternifolia oil-based nanoemulsions in cosmetic, food, and pharma sectors has been discussed.