结直肠癌（CRC）是当今世界上常见的恶性肿瘤，肝转移是导致CRC患者死亡的主要原因。虽然溶酶体整合膜蛋白2（LIMP2）在胃癌和前列腺癌中被报道发挥重要作用，但其在CRC中的作用尚不清楚。本研究的目的是调查LIMP2在CRC侵袭和迁移中的功能，以及潜在的分子机制。我们发现，与癌旁正常组织相比，CRC组织中LIMP2水平较高。Kaplan-Meier生存分析显示，LIMP2的高表达与CRC患者的预后较差相关。LIMP2的沉默显著抑制了CRC细胞的体外侵袭、迁移和伤口愈合能力，并抑制了CRC的肝转移。此外，LIMP2的沉默也抑制了CRC中的自噬作用。因此，LIMP2在CRC的进展中发挥重要作用。LIMP2的高表达与CRC患者的预后较差相关。沉默LIMP2可以有效抑制体外CRC细胞的迁移和侵袭，并预防体内的肝转移。这些发现表明LIMP2可能作为独立的预后因子和CRC的潜在治疗靶点。版权所有 © 2023 Elsevier Inc.

Colorectal cancer (CRC) is a common malignancy worldwide nowadays and liver metastasis is the primary cause of death in patients with CRC. Although lysosomal integral membrane protein 2 (LIMP2) has been reported to play important roles in gastric cancer and prostate cancer, its role in CRC remains unclear. The aim of this study was to investigate the function of LIMP2 in CRC invasion and migration, along with the potential underlying molecular mechanisms. We found that LIMP2 levels were higher in CRC tissues compared to adjacent normal tissues. Kaplan-Meier survival analysis showed that high expression of LIMP2 was associated with worse prognosis in CRC patients. Knockdown of LIMP2 significantly inhibited invasion, migration, and wound healing abilities of CRC cells in vitro, and inhibited CRC liver metastasis in vivo. Additionally, LIMP2 knockdown inhibited autophagy in CRC. Therefore, LIMP2 plays an important role in CRC progression. High expression of LIMP2 was associated with worse prognosis in CRC patients. Knockdown LIMP2 can effectively inhibit CRC cell migration and invasion in vitro and prevent liver metastasis in vivo. These findings suggest that LIMP2 may serve as an independent prognostic factor and potential therapeutic target for CRC.Copyright © 2023. Published by Elsevier Inc.