炎性反应现象引起早产(PTD)。 硫化氢是一种气体传递物质，具有抗炎特性，通过胱氨酸-γ-裂解酶(CSE)酶的活性产生，其影响在小鼠正常分娩和PTD模型中进行了研究。将雌性CSE+/+和CSE-/-小鼠与雄性CSE+/+小鼠交配；交配过程在喝水未补充和补充半胱氨酸的情况下进行。监测妊娠率。在妊娠的第14.5天通过腹腔注射细菌脂多糖(LPS)诱导PTD。小鼠在6小时和12小时后进行组织采集和脾细胞分离。分离的脾细胞通过刺激产生肿瘤坏死因子-α (TNFα)，白细胞介素(IL)-10和干扰素-γ(IFNγ)；测量胎儿和胎盘中的TNFα和血管内皮生长因子(VEGF)。CSE-/-小鼠的成功妊娠率较低，通过半胱氨酸补充恢复。CSE缺陷与胎儿组织中TNFα浓度升高、IL-10反应减弱和脾细胞产生IFNγ增加相关。CSE缺陷与PTD无关。在PTD诱导后，CSE-/-小鼠的IL-10反应未减弱，但TNFα和IFNγ的产生随时间降低；胎盘VEGF也随时间增加。CSE缺陷对妊娠产生不利影响。通过CSE缺陷的H2S缺陷并不导致PTD, 但在胎儿中介导炎性反应现象。 © 2023 The Authors. 《American Journal of Reproductive Immunology published by John Wiley & Sons Ltd.》

Pro-inflammatory phenomena drive preterm delivery (PTD). Hydrogen sulfide is a gasotransmitter with anti-inflammatory properties produced through the activity of the enzyme cystathionine-γ-lyase (CSE), and its impact was studied in models of normal delivery and PTD in mice.Female CSE+/+ and CSE-/- mice were mated with male CSE+/+ mice; mating was done with drinking water unsupplemented and supplemented with cysteine. The pregnancy rate was monitored. PTD was induced by the intraperitoneal injection of bacterial lipopolysaccharide (LPS) on day 14.5 of pregnancy. Mice were sacrificed for tissue collection and splenocyte isolation after 6 and 12 h. Isolated splenocytes were stimulated for the production of tumor necrosis factor-alpha (TNFα), interleukin (IL)-10 and interferon-gamma (IFNγ); TNFα and vascular endothelial growth factor (VEGF) were measured in the fetuses and the placenta.The successful pregnancy rate was lower in CSE-/- mice and it was restored with cysteine supplementation. CSE deficiency was associated with higher tissue concentrations of TNFα in the fetuses, attenuated IL-10 responses and higher IFNγ production from splenocytes. CSE deficiency was not associated with PTD. Following PTD induction, CSE-/- mice did not show attenuated IL-10 responses but the production of TNFα and IFNγ was lowered over-time; placental VEGF was also increased over-time.CSE deficiency has an unfavorable impact on pregnancy. H2 S deficiency through CSE does not drive PTD but mediates pro-inflammatory phenomena in fetuses.© 2023 The Authors. American Journal of Reproductive Immunology published by John Wiley & Sons Ltd.