内生真菌产生一系列已知的代谢物和其他几种尚未探索的代谢物，从药物和工业的角度来看，它们具有重要的生物活性。虽然有几项研究报道了咖啡（Coffea arabica）植物内生菌的多样性，但只有少数在有机培养条件下进行了描述。在本文中，我们描述了与咖啡（Coffea arabica L.）（茜草科）相关的内生真菌Siamese收斗壳属（Colletotrichum siamense）菌株乙酸乙酯相中专门代谢物的化学特征，并评估了其对医学和食品中重要的肿瘤细胞和细菌的潜力。通过MTT法和稀释培养基微孔法进行肿瘤细胞MCF-7和HepG2/C3A的细胞毒性实验评估代谢物提取物的抗菌作用。抗增殖实验结果显示，在处理后的24小时内，两种细胞类型的50%抑制浓度表现出了很好的效果。使用带有电喷雾电离源的超高效液相色谱-串联质谱（UHPLC-MS/MS）分析了提取物，并鉴定了Siamese收斗壳属菌的化合物，该种类作为活性代谢物资源仍未得到充分探索。在内生真菌中观察到的许多化合物需要在工业上进行化学合成，成本较高，而由真菌生产则成为一种在化学和经济上更可行的替代方案。在与肿瘤细胞对抗的不同机制中，检测到了间苯二酚、香树脂醇和α-亚麻酸等主要化合物。Siamese收斗壳属内生真菌提取物具有多种具有药理潜力和抗菌活性的化合物，证实了其在生物技术应用中的潜力。©2023 版权所有, 授权给巴西微生物学会。

Endophytic fungi produce a range of known metabolites and several others, not yet explored, which present important biological activities from the pharmaceutical and industrial perspective. Several studies have reported the diversity of endophytes in Coffea arabica plants, although few have been described in organic cultures. In the current paper, we describe the chemical profile of specialized metabolites in the ethyl acetate phase in a strain of the endophytic fungus Colletotrichum siamense associated with coffee (Coffea arabica L.) (Rubiaceae) and its potential against tumor cells and bacteria of medical and food importance. Cytotoxicity assays in tumor cells MCF-7 and HepG2/C3A were performed by MTT and microdilution in broth to evaluate the antibacterial action of metabolic extract. The antiproliferative assay showed promising results after 24 h of treatment, with 50% injunction concentrations for the two cell types. UHPLC-MS/MS analyses with an electrospray ionization source were used to analyze the extracts and identify compounds of species Colletotrichum siamense, which is still little explored as a source of active metabolites. Many of these compounds observed in the endophytic need to be chemically synthesized in industry, at high costs, while production by the fungus becomes a chemically and economically more viable alternative. Pyrocatechol, gentisyl alcohol, and alpha-linolenic acid, associated with different mechanisms of action against tumor cells, were detected among the main compounds. The extract of the endophytic fungus Colletotrichum siamense presented several compounds with pharmacological potential and antibacterial activity, corroborating its potential in biotechnological applications.© 2023. The Author(s) under exclusive licence to Sociedade Brasileira de Microbiologia.