非线性显微镜是一种强大的成像工具，可用于检查各种生物样品的结构特性和亚细胞过程。第三次谐波产生（THG）的能力包括无标记成像，分辨率为衍射极限，并具有几乎无光毒性的三维可视化效果。在本研究中，我们利用THG记录和量化果蝇血细胞对正常或肿瘤性神经细胞的脂质含量，并与其外形或状态进行相关。我们发现成年血细胞在遇到正常细胞之前和之后的脂质积累是相似的。相反，在成年血细胞与癌细胞相互作用之前，脂质指数较低，但在其参与吞噬作用时增加， exhaust 后再次减少。血细胞在遇到肿瘤细胞时脂质积累的动态变化可能是评估肿瘤相关血细胞吞噬能力或活化状态的有用工具。本文受版权保护。保留所有权利。

Non-linear microscopy is a powerful imaging tool to examine structural properties and subcellular processes of various biological samples. The competence of Third Harmonic Generation (THG) includes the label free imaging with diffraction-limited resolution and three- dimensional visualization with negligible phototoxicity effects. In this study, THG records and quantifies the lipid content of Drosophila haemocytes, upon encountering normal or tumorigenic neural cells, in correlation with their shape or their state. We show that the lipid accumulations of adult haemocytes are similar before and after encountering normal cells. In contrast, adult haemocytes prior to their interaction with cancer cells have a low lipid index, which increases while they are actively engaged in phagocytosis only to decrease again when haemocytes become exhausted. This dynamic change in the lipid accrual of haemocytes upon encountering tumour cells could potentially be a useful tool to assess the phagocytic capacity or activation state of tumour-associated haemocytes. This article is protected by copyright. All rights reserved.This article is protected by copyright. All rights reserved.